Lyn Antibody - #AF6119

Fig. 9 Expression of critical ERSRGs associated with advanced atherosclerotic plaque in atherosclerotic tissues (a) Representative images of HE, and Oil Red O staining of aortic root sections of ApoE−/− mice. Scale bar, 200 μm (n = 3). (b) Quantification of plaque areas, lipid areas of aortic root sections of ApoE−/− mice. (n = 3). (c) mRNA expression level of BIP, and CHOP in the aorta from ApoE–/– mice fed a WD and ND for 16 weeks. (n = 3). (d) mRNA expression level of CTSB, LYN, and CYBB in the aorta from ApoE–/– mice fed a WD and ND for 16 weeks. (n = 3). (e) Western blot analysis of CTSB, LYN, and CYBB protein levels in the aorta from ApoE–/– mice fed a WD and ND for 16 weeks (n = 3). GAPDH was used as the internal control. The grouping of gels/blots cropped from different gels. Uncropped blots are available in Supplementary Fig. S4. (f) Levels of CYBB, LYN, CTSB (green), and CD68 (red) in advanced and early atherosclerotic plaque regions; merged images (yellow) show more puncta in advanced atherosclerotic plaque regions. Scale bar: 200 μm, 100 μm, 50 μm. (g) ApoE–/– mice were fed WD or ND for 16 weeks. Immunofluorescence analysis of CTSB, LYN, CYBB (green), and CD68 (red) expression in aortic root sections. Merged images (yellow) show more puncta in aortic root sections from mice were fed WD for16 weeks. Scale bar: 200 μm, 50 μm, 20μm (n = 3). ND normal diet, WD western diet, BMDM bone marrow derived macrophage. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.