产品: 磷酸化 RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) 抗体
货号: AF3810
描述: Rabbit polyclonal antibody to Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232)
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat, Monkey
蛋白号: Q15418 | P51812 | Q15349 | Q9UK32
RRID: AB_2847124

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/s218/232.
RRID:
AB_2847124
引用格式: Affinity Biosciences Cat# AF3810, RRID:AB_2847124.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

90 kDa ribosomal protein S6 kinase 3; CLS; HU 3; HU2; HU3; Insulin stimulated protein kinase 1; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A3_HUMAN; MAP kinase activated protein kinase 1b; MAP kinase-activated protein kinase 1b; MAPK activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK 1b; MAPKAPK-1b; MAPKAPK1B; Mental retardation, X linked 19; MRX19; OTTHUMP00000023036; p90 RSK2; p90 RSK3; p90-RSK 3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal protein S6 kinase alpha-3; Ribosomal protein s6 kinase ii alpha 2; Ribosomal S6 kinase 2; Rps6ka3; RSK; RSK-2; RSK2; S6 kinase 2; S6K alpha3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK

抗原和靶标

免疫原:

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/s218/232.

基因/基因ID:

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). Activation of RSK2 upregulates SOX8 to promote methotrexate resistance in gestational trophoblastic neoplasia. LABORATORY INVESTIGATION, 2021 (PubMed: 34373588) [IF=5.1]

Application: WB    Species: Human    Sample: JAR/MTX and JAR cell

Fig. 1Quantitative phosphoproteomic analysis on JAR/MTX and JAR cell models.

Application: IHC    Species: Human    Sample: GTN

Fig. 6RSK2/SOX8 signaling was highly enriched in MTX-resistant GTN samples. A Immunostaining of p-RSK and SOX8 in GTN samples. Representative IHC staining micrographs were shown for MTX-resistant (left panel) or MTX-sensitive (right panel) GTNs. Bars: 100 µm. B p-RSK and SOX8 were highly expressed in MTX-resistant GTN specimens. *P < 0.05; **P < 0.01. C High expression of p-RSK was significantly correlated with high expression of SOX8 in GTN specimens.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.