Phospho-CaMKII alpha (Ser331) Antibody - #AF3793

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产品: 磷酸化 CaMKII alpha (Ser331) 抗体
货号: AF3793
描述: Rabbit polyclonal antibody to Phospho-CaMKII alpha (Ser331)
应用: IF/ICC
反应: Human, Mouse, Rat
分子量: 54kD(Calculated).
蛋白号: Q9UQM7
RRID: AB_2847107

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-CaMK2 alpha (Ser331) Antibody detects endogenous levels of CaMK2 alpha only when phosphorylated at Ser331.
RRID:
AB_2847107
引用格式: Affinity Biosciences Cat# AF3793, RRID:AB_2847107.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha CaMKII; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha B subunit; Calcium/calmodulin dependent protein kinase type II alpha chain; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMK II alpha subunit; CaMK-II subunit alpha; Camk2a; CAMKA; CaMKII; CaMKIINalpha; EC 2.7.11.17; KCC2A_HUMAN; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320;

抗原和靶标

免疫原:

A synthesized peptide derived from human CaMK2 alpha around the phosphorylation site of Ser331.

Uniprot:

Q9UQM7(KCC2A_HUMAN) >>Visit HPA database.

基因/基因ID:
CAMK2A(815)
序列:
MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH

翻译修饰 - Q9UQM7 作为底物

Site PTM Type Enzyme
C6 S-Nitrosylation
Y13 Phosphorylation
K56 Ubiquitination
K68 Ubiquitination
S78 Phosphorylation
K137 Ubiquitination
S145 Phosphorylation
K226 Acetylation
K226 Ubiquitination
Y230 Phosphorylation
S234 Phosphorylation
K245 Ubiquitination
T253 Phosphorylation
K258 Methylation
C280 S-Nitrosylation
T286 Phosphorylation Q9UQM7 (CAMK2A)
K298 Methylation
K300 Methylation
T305 Phosphorylation Q9UQM7 (CAMK2A)
T306 Phosphorylation Q9UQM7 (CAMK2A)
T310 Phosphorylation
S314 Phosphorylation Q9UQM7 (CAMK2A)
S318 Phosphorylation
S331 Phosphorylation
S333 Phosphorylation
T334 Phosphorylation
T336 Phosphorylation
T337 Phosphorylation

翻译修饰 - Q9UQM7 作为激酶

Substrate Site Source
A8K161 (CAMK2A) T286 Uniprot
A8K161 (CAMK2A) T305 Uniprot
O00631 (SLN) T5 Uniprot
O14490-2 (DLGAP1) S44 Uniprot
O14713-2 (ITGB1BP1) T38 Uniprot
O15273 (TCAP) S157 Uniprot
O15273 (TCAP) S161 Uniprot
O95999 (BCL10) S138 Uniprot
P00533 (EGFR) S768 Uniprot
P00533-1 (EGFR) S1064 Uniprot
P00533 (EGFR) S1070 Uniprot
P00533 (EGFR) S1071 Uniprot
P00533-1 (EGFR) S1081 Uniprot
P00533-1 (EGFR) S1120 Uniprot
P00533 (EGFR) S1166 Uniprot
P04626 (ERBB2) T1172 Uniprot
P05556 (ITGB1) T788 Uniprot
P05556 (ITGB1) T789 Uniprot
P05783 (KRT18) S53 Uniprot
P07101 (TH) S19 Uniprot
P07101-4 (TH) S35 Uniprot
P07101-3 (TH) S40 Uniprot
P07101-4 (TH) S44 Uniprot
P07101 (TH) S71 Uniprot
P08670 (VIM) S83 Uniprot
P09917 (ALOX5) S272 Uniprot
P10636-6 (MAPT) S204 Uniprot
P10636-8 (MAPT) S262 Uniprot
P10636-2 (MAPT) S267 Uniprot
P10636-6 (MAPT) S298 Uniprot
P10636-8 (MAPT) S356 Uniprot
P10636-8 (MAPT) S416 Uniprot
P10636 (MAPT) T548 Uniprot
P10636 (MAPT) S552 Uniprot
P10636 (MAPT) S579 Uniprot
P11831 (SRF) S103 Uniprot
P11831 (SRF) T160 Uniprot
P14136-1 (GFAP) S13 Uniprot
P14136-1 (GFAP) S17 Uniprot
P14136-1 (GFAP) S38 Uniprot
P14136-1 (GFAP) S393 Uniprot
P14921 (ETS1) S251 Uniprot
P14921 (ETS1) S257 Uniprot
P14921-1 (ETS1) S282 Uniprot
P14921 (ETS1) S285 Uniprot
P15036 (ETS2) S246 Uniprot
P15036 (ETS2) S310 Uniprot
P15036 (ETS2) S313 Uniprot
P16070 (CD44) S706 Uniprot
P16220 (CREB1) S142 Uniprot
P16615-2 (ATP2A2) S38 Uniprot
P16949 (STMN1) S16 Uniprot
P17676-1 (CEBPB) S325 Uniprot
P18433 (PTPRA) S204 Uniprot
P20941-2 (PDC) S2 Uniprot
P20941-2 (PDC) S21 Uniprot
P20941-1 (PDC) S54 Uniprot
P20941-1 (PDC) S73 Uniprot
P21333 (FLNA) S2523 Uniprot
P23677 (ITPKA) T311 Uniprot
P26678 (PLN) T17 Uniprot
P28329-3 (CHAT) T456 Uniprot
P28329 (CHAT) T574 Uniprot
P29475 (NOS1) S852 Uniprot
P31645 (SLC6A4) S13 Uniprot
P35222 (CTNNB1) T332 Uniprot
P35222 (CTNNB1) T472 Uniprot
P35222 (CTNNB1) S552 Uniprot
P35270 (SPR) S213 Uniprot
P35367 (HRH1) T140 Uniprot
P35367 (HRH1) T142 Uniprot
P35367 (HRH1) S396 Uniprot
P35367 (HRH1) S398 Uniprot
P35372 (OPRM1) S268 Uniprot
P35372 (OPRM1) S270 Uniprot
P35372 (OPRM1) T372 Uniprot
P35462 (DRD3) S229 Uniprot
P37840 (SNCA) S129 Uniprot
P42261 (GRIA1) S567 Uniprot
P42261 (GRIA1) S832 Uniprot
P46527 (CDKN1B) S10 Uniprot
P48058 (GRIA4) S862 Uniprot
P51790 (CLCN3) S109 Uniprot
P55042 (RRAD) S273 Uniprot
P55055 (NR1H2) S426 Uniprot
Q00613-1 (HSF1) S230 Uniprot
Q00975 (CACNA1B) S2120 Uniprot
Q01970 (PLCB3) S537 Uniprot
Q04760 (GLO1) T107 Uniprot
Q05397 (PTK2) S843 Uniprot
Q05469 (LIPE) S855 Uniprot
Q05682 (CALD1) S643 Uniprot
Q05682 (CALD1) S656 Uniprot
Q06210 (GFPT1) S261 Uniprot
Q12879-1 (GRIN2A) S1291 Uniprot
Q12959 (DLG1) S232 Uniprot
Q13224 (GRIN2B) S383 Uniprot
Q13224 (GRIN2B) S1303 Uniprot
Q14653 (IRF3) S386 Uniprot
Q14654 (KCNJ11) T180 Uniprot
Q14654 (KCNJ11) T224 Uniprot
Q14674 (ESPL1) S1501 Uniprot
Q15121 (PEA15) S116 Uniprot
Q15796 (SMAD2) S110 Uniprot
Q15796-1 (SMAD2) S240 Uniprot
Q15796-1 (SMAD2) S260 Uniprot
Q4G163 (FBXO43) T234 Uniprot
Q86UR5 (RIMS1) S242 Uniprot
Q86UR5 (RIMS1) S288 Uniprot
Q8IZP0 (ABI1) S88 Uniprot
Q92736 (RYR2) S2808 Uniprot
Q92736 (RYR2) S2814 Uniprot
Q96A00 (PPP1R14A) S130 Uniprot
Q96NW7 (LRRC7) S1439 Uniprot
Q96PH1-4 (NOX5) S475 Uniprot
Q96PM5 (RCHY1) T154 Uniprot
Q96PM5 (RCHY1) S155 Uniprot
Q96PV0-1 (SYNGAP1) S1073 Uniprot
Q96PV0-1 (SYNGAP1) T1077 Uniprot
Q96PV0-1 (SYNGAP1) S1138 Uniprot
Q9BXL7 (CARD11) S116 Uniprot
Q9NSC5 (HOMER3) S120 Uniprot
Q9NSC5 (HOMER3) S159 Uniprot
Q9NSC5 (HOMER3) S176 Uniprot
Q9UBS5 (GABBR1) S868 Uniprot
Q9UQL6 (HDAC5) S259 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot
Q9UQM7-1 (CAMK2A) T286 Uniprot
Q9UQM7-1 (CAMK2A) T305 Uniprot
Q9UQM7 (CAMK2A) T306 Uniprot
Q9UQM7 (CAMK2A) S314 Uniprot
Q9Y496 (KIF3A) S687 Uniprot
Q9Y4D2 (DAGLA) S782 Uniprot
Q9Y4D2 (DAGLA) S808 Uniprot

研究背景

功能:

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity.

翻译修饰:

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.

细胞定位:

Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Dendrite.
Note: Postsynaptic lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with BAALC. Interacts with MPDZ. Interacts with SYN1. Interacts with CAMK2N2. Interacts with SYNGAP1. Interacts with SYNPO2 (By similarity). Interacts with SHANK3. Interacts with GRIN2B. Interacts with CACNB2. Interacts with LRRC7. Interacts with GRM5.

蛋白家族:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

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