Phospho-DDX3 (Ser102) Antibody - #AF3782

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产品: 磷酸化 DDX3 (Ser102) 抗体
货号: AF3782
描述: Rabbit polyclonal antibody to Phospho-DDX3 (Ser102)
应用: WB
文献验证: WB
反应: Human
蛋白号: O00571
RRID: AB_2847096

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
Phospho-DDX3 (Ser102) Antibody detects endogenous levels of DDX3 only when phosphorylated at Ser102.
RRID:
AB_2847096
引用格式: Affinity Biosciences Cat# AF3782, RRID:AB_2847096.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP dependent RNA helicase DDX3X; ATP-dependent RNA helicase DDX3X; CAP Rf; DBX; DDX14; DDX3X; DDX3X_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked; DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked; DEAD box; DEAD box protein 3; DEAD box protein 3 X-chromosomal; DEAD box X isoform; DEAD box, X isoform; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3; DEAD/H box 3; DEAD/H box 3, X-linked; Helicase like protein 2; Helicase-like protein 2; HLP2; X isoform; X-chromosomal;

抗原和靶标

免疫原:

A synthesized peptide derived from human DDX3 around the phosphorylation site of Ser102.

基因/基因ID:
DDX3X(1654)

研究领域

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

文献引用

1). AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer. Autophagy, 2024 (PubMed: 37733921) [IF=14.6]

Application: WB    Species: Human    Sample: MDA-MB-231 WT and RB1CC1 KO cells

Figure 5. DDX3X interacts with AZI2 and contributes to the expression of pro-inflammatory genes upon TBK1 activation in RB1CC1 depleted cells. (A) immunoblots of GFP and DDX3X showing levels of these proteins in inputs and IP:GFP fractions from Ctrl -AZI2, Ctrl +AZI2 and RB1CC1 KO +AZI2 cells. (B) immunoblots showing levels of RB1CC1, p-DDX3X (S102), DDX3X and ACTB in MDA-MB-231 WT and RB1CC1 KO cells. (C) immunoblots of DDX3X and IRF3 showing levels of these proteins in inputs and IP:DDX3X fractions from Ctrl -AZI2, RB1CC1 KO -AZI2, Ctrl +AZI2 and RB1CC1 KO +AZI2 cells. (D) confocal imaging of GFP-AZI2 puncta and DDX3X localization in Ctrl or RB1CC1 KO +AZI2 cells. Scale bar: 10 µm. (E) immunoblots showing levels of RB1CC1, DDX3X, IRF3, VCL and PARP1 in cytoplasmic and nuclear extracts from Ctrl or RB1CC1 KO +AZI2 cells. (F) immunoblots showing levels of RB1CC1, DDX3X and GAPDH in Ctrl or RB1CC1 KO +AZI2 cells. (G) bar charts showing relative mRNA levels of Cxcl9, Cxcl10 and Ccl5 normalized to Actb expression in Ctrl or RB1CC1 KO +AZI2 cells and respective shRnas.

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