产品: ATP1A1 抗体
货号: AF6109
描述: Rabbit polyclonal antibody to ATP1A1
应用: WB IF/ICC
反应: Human, Mouse, Rat
分子量: 113kDa; 113kD(Calculated).
蛋白号: P05023
RRID: AB_2834996

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
ATP1A1 Antibody detects endogenous levels of total ATP1A1.
RRID:
AB_2834996
引用格式: Affinity Biosciences Cat# AF6109, RRID:AB_2834996.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A1A1; AT1A1; AT1A1_HUMAN; ATP1A1; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting subunit alpha 1; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+; K+ ATPase alpha subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na;K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
ATP1A1 the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients.
序列:
MGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGFSMLLWIGAILCFLAYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVDWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

翻译修饰 - P05023 作为底物

Site PTM Type Enzyme
K9 Ubiquitination
Y10 Phosphorylation
S16 Phosphorylation Q05513 (PRKCZ) , P28482 (MAPK1)
K21 Ubiquitination
K36 Ubiquitination
K37 Ubiquitination
K45 Ubiquitination
S47 Phosphorylation
K54 Ubiquitination
Y55 Phosphorylation
S60 Phosphorylation
R61 Methylation
T81 Phosphorylation
T85 Phosphorylation
T86 Phosphorylation
Y149 Phosphorylation
K156 Ubiquitination
K162 Ubiquitination
K177 Ubiquitination
K194 Ubiquitination
R198 Methylation
R204 Methylation
S207 Phosphorylation
K212 Ubiquitination
S216 Phosphorylation
S217 Phosphorylation
S222 Phosphorylation
T226 Phosphorylation
S228 Phosphorylation
S246 Phosphorylation
C249 S-Nitrosylation
Y260 Phosphorylation
T261 Phosphorylation
R264 Methylation
T265 Phosphorylation
K359 Ubiquitination
S369 Phosphorylation
K377 Ubiquitination
T378 Phosphorylation
S407 Phosphorylation
K444 Ubiquitination
S452 Phosphorylation
K458 Ubiquitination
C459 S-Nitrosylation
C463 S-Nitrosylation
S466 Phosphorylation
K468 Ubiquitination
K476 Ubiquitination
S484 Phosphorylation
K487 Acetylation
K487 Ubiquitination
Y488 Phosphorylation
K494 Methylation
K494 Ubiquitination
T498 Phosphorylation
S499 Phosphorylation
K508 Ubiquitination
R517 Methylation
S520 Phosphorylation
K526 Sumoylation
K526 Ubiquitination
K535 Ubiquitination
Y542 Phosphorylation
K605 Ubiquitination
K612 Ubiquitination
T617 Phosphorylation
T623 Phosphorylation
K625 Ubiquitination
K629 Ubiquitination
T640 Phosphorylation
S653 Phosphorylation
K661 Ubiquitination
K671 Ubiquitination
K683 Ubiquitination
Y684 Phosphorylation
T686 Phosphorylation
K698 Ubiquitination
C705 S-Nitrosylation
S722 Phosphorylation
K726 Ubiquitination
K727 Ubiquitination
K773 Ubiquitination
K833 Ubiquitination
K840 Ubiquitination
K843 Ubiquitination
Y902 Phosphorylation
S943 Phosphorylation P17612 (PRKACA)
K950 Ubiquitination
K952 Ubiquitination
K1019 Ubiquitination

研究背景

功能:

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

翻译修饰:

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).

细胞定位:

Cell membrane>Sarcolemma>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3. Interacts with SIK1 (By similarity). Binds the HLA class II histocompatibility antigen DR1. Interacts with SLC35G1 and STIM1.

蛋白家族:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Lv Y et al. Aldosterone down-regulates the slowly activated delayed rectifier potassium current in adult guinea pig cardiomyocytes. British Journal of Pharmacology 2015 Dec;172(23):5596-608 (PubMed: 25857626) [IF=7.3]

Application: WB    Species:    Sample:


2). Cui L et al. Real-Ambient Particulate Matter Exposure-Induced Cardiotoxicity in C57/B6 Mice. Frontiers in Pharmacology 2020 Mar 31;11:199 (PubMed: 32296328) [IF=5.6]

Application: WB    Species: Mice    Sample: heart tissues

Figure 5 Western blotting results. For 6 weeks, 6–8 weeks C57/B6 mice, with or without Nrf2 knockout, were exposed to either filtered air or air containing particulate matter (PM), and then the hearts were collected, snap-frozen in liquid nitrogen, extracted, and subjected to western blotting for p-SMAD2, SMAD2, p-p38MAPK, p38MAPK, p-TAK1, TAK1, ATP1a1, Grb2, p-JAK1, JAK1, p-STAT3, STAT3, and GAPDH. An additional group of WTC and WTE heart samples was processed with nuclear and cytoplasmic extraction kit (Epizyme, Shanghai, China) and probed for Nrf2 and GAPDH. N = 3 per group from three independent experiments. *Statistically different between the two groups (P < 0.05). **Statistically different between the two groups (P < 0.01).

3). Qu F et al. Comparison of the effects of green and black tea extracts on Na+ /K+ -ATPase activity in intestine of type 1 and type 2 diabetic mice. MOLECULAR NUTRITION & FOOD RESEARCH 2019 Jun 13:e1801039 (PubMed: 31197949) [IF=5.2]

Application: WB    Species: mouse    Sample: intestine

Figure 3.| Effect of green and black tea extracts on Na+/K+-ATPase in intestine. A) Activity of Na+/K+-ATPase in the intestine in type 1 diabetic mice model. B) Activity of Na+/K+-ATPase in the intestine in type 2 diabetic mice model. C) Protein expression of Na+/K+-ATPase in the intestine in type 1 diabetic mice model.

4). Ding C et al. QiShenYiQi pills, a Chinese patent medicine, increase bioavailability of atorvastatin by inhibiting Mrp2 expression in rats. PHARMACEUTICAL BIOLOGY 2022 Dec;60(1):185-194. (PubMed: 35001796) [IF=3.8]

Application: WB    Species: Rat    Sample: ileum

Figure 6. Protein expression of P-glycoprotein in jejunum and Mrp2 in ileum after oral or intravenous administration of ATV without or with QSYQ. (A) and (B) Protein expression of P-glycoprotein in jejunum; (C) and (D) Protein expression of Mrp2 in ileum. Data are shown as mean ± SD (n = 3). *p < 0.05 compared to without QSYQ.

5). Li X et al. Endogenous acetylcholine increases alveolar epithelial fluid transport via activation of alveolar epithelial Na,K-ATPase in mice. RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY 2015 Oct;217:25-31 (PubMed: 26134533) [IF=2.3]

Application: IF/ICC    Species: human    Sample: A549 cells


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