Progesterone Receptor Antibody - #AF6106

Fig. 3. The expression of SGK1, ENaC-α, ERβ and PR in decidua tissue was detected by immunohistochemistry. Note: vs Control group, ***P < 0.001. Scale bars: a 100 μm, b 50 μm (n = 10).

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice. (A) WT C57/BL6J mice were pretreated with PTS (10 mg/kg) and afterwards with DOX (20 mg/kg cumulative dose) for 6 days. Immunochemistry staining of the liver sections with anti-NLRP3 (left, scale bar = 50 μm), the quantification of NLRP3 positive area in each group (right, n = 6); (B) qPCR analyses of NLRP3, IL-1β and IL-18 mRNA levels in each group (n = 6); (C) Western blot analyses of NLRP3, ASC, Caspase-1 p20, IL-1β, and IL-18 proteins and the quantification of the blots in each group (n = 4 per group); (D) Western blot analyses of Cleaved Caspase-3, BAX, and BCL-2 proteins (left) and quantification of the blots in each group (right, n = 4 per group).

Figure 4 RMC-4550 inhibits the nuclear translocation of PR and the expression of PR target genes in hESCs treated with P4. (A–B) Immunofluorescent staining of PR (red) in hESCs stimulated with P4 (2  μM) with or without RMC-4550 treatment for 1 h. Nuclei were stained with DAPI (blue). The number of nuclear PR cells from five different fields was counted and plotted as a histogram. Bar figures represent the ratios of nuclear PR. Scale bar = 50 µm; 10 μm (magnified graphs). (C–D) Immunofluorescent staining of PR (red) in hESCs, in which stimulated with P4 (2 μM) for 1 h, after transfection with sh-SHP2 or sh-Ctrl for 72 h. Nuclei were stained with DAPI (blue). The number of nuclear PR cells from five different fields was counted and plotted as a histogram. Bar figures represent the ratios of nuclear PR. Scale bar = 50 µm; 10 μm (magnified graphs). (E–H) Real-time PCR analysis of the transcriptional levels of the PR target genes HAND2, PLZF, HSD11B, and BMP2 in hESCs treated with or without P4 with or without RMC-4550. Error bars denote the s.d. (n = 3). Data were calibrated to the most highly expressing sample in blank control (yellow color).