| 产品: | 磷酸化 IRF2 (Ser225) 抗体 |
| 货号: | AF3723 |
| 描述: | Rabbit polyclonal antibody to Phospho-IRF2 (Ser225) |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse |
| 分子量: | 39kD(Calculated). |
| 蛋白号: | P14316 |
| RRID: | AB_2847037 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3723, RRID:AB_2847037.
展开/折叠
DKFZp686F0244; Interferon regulatory factor 2; IRF 2; IRF-2; IRF2; IRF2_HUMAN;
抗原和靶标
A synthesized peptide derived from human IRF2 around the phosphorylation site of Ser225.
Expressed throughout the epithelium of the colon. Also expressed in lamina propria.
- P14316 IRF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPVERMRMRPWLEEQINSNTIPGLKWLNKEKKIFQIPWMHAARHGWDVEKDAPLFRNWAIHTGKHQPGVDKPDPKTWKANFRCAMNSLPDIEEVKDKSIKKGNNAFRVYRMLPLSERPSKKGKKPKTEKEDKVKHIKQEPVESSLGLSNGVSDLSPEYAVLTSTIKNEVDSTVNIIVVGQSHLDSNIENQEIVTNPPDICQVVEVTTESDEQPVSMSELYPLQISPVSSYAESETTDSVPSDEESAEGRPHWRKRNIEGKQYLSNMGTRGSYLLPGMASFVTSNKPDLQVTIKEESNPVPYNSSWPPFQDLPLSSSMTPASSSSRPDRETRASVIKKTSDITQARVKSC
翻译修饰 - P14316 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K29 | Acetylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K75 | Acetylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Sumoylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K137 | Sumoylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| K166 | Sumoylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K260 | Sumoylation | Uniprot | |
| K293 | Sumoylation | Uniprot | |
| S333 | Phosphorylation | Uniprot |
研究背景
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
Nucleus.
Expressed throughout the epithelium of the colon. Also expressed in lamina propria.
Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity.
Belongs to the IRF family.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.