产品: Lck 抗体
货号: AF6101
描述: Rabbit polyclonal antibody to Lck
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 56kDa; 58kD(Calculated).
蛋白号: P06239
RRID: AB_2834990

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(82%)
克隆:
Polyclonal
特异性:
Lck Antibody detects endogenous levels of total Lck.
RRID:
AB_2834990
引用格式: Affinity Biosciences Cat# AF6101, RRID:AB_2834990.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IMD22; LCK; Lck p56; LCK proto-oncogene, Src family tyrosine kinase; LCK_HUMAN; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell specific protein tyrosine kinase; Lymphocyte cell-specific protein-tyrosine kinase; Lymphocyte specific protein tyrosine kinase; Membrane associated protein tyrosine kinase; Oncogene lck; P56 LCK; p56(LSTRA) protein tyrosine kinase; p56-LCK; p56lck; pp58 lck; pp58lck; Protein YT16; Proto oncogene tyrosine protein kinase LCK; Proto-oncogene Lck; Protooncogene tyrosine protein kinase LCK; T cell specific protein tyrosine kinase; T cell-specific protein-tyrosine kinase; T lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Lck; YT 16; YT16;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P06239 LCK_HUMAN:

Expressed specifically in lymphoid cells.

描述:
Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function.
序列:
MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
91
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P06239 作为底物

Site PTM Type Enzyme
Y25 Phosphorylation
T35 Phosphorylation
S42 Phosphorylation P27361 (MAPK3) , P17252 (PRKCA) , P17612 (PRKACA) , P28482 (MAPK1)
T50 Phosphorylation
Y51 Phosphorylation
S59 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
K84 Ubiquitination
S94 Phosphorylation
K99 Ubiquitination
S102 Phosphorylation
K118 Ubiquitination
S121 Phosphorylation
K130 Ubiquitination
S133 Phosphorylation
S150 Phosphorylation
S156 Phosphorylation
S158 Phosphorylation
T159 Phosphorylation
S162 Phosphorylation
S166 Phosphorylation Q02156 (PRKCE)
K179 Acetylation
K179 Ubiquitination
Y181 Phosphorylation
K182 Ubiquitination
Y192 Phosphorylation P43405 (SYK)
S194 Phosphorylation
T198 Phosphorylation
Y209 Phosphorylation
T210 Phosphorylation
S213 Phosphorylation
K228 Ubiquitination
K231 Ubiquitination
K246 Ubiquitination
Y263 Phosphorylation
Y264 Phosphorylation
T268 Phosphorylation
K269 Ubiquitination
K273 Ubiquitination
S274 Phosphorylation
K276 Ubiquitination
S281 Phosphorylation
K293 Ubiquitination
K335 Ubiquitination
T375 Phosphorylation
K379 Ubiquitination
Y394 Phosphorylation P06239 (LCK)
T395 Phosphorylation
K401 Ubiquitination
K405 Ubiquitination
Y414 Phosphorylation
T416 Phosphorylation
Y470 Phosphorylation
K478 Ubiquitination
Y489 Phosphorylation
S492 Phosphorylation
T501 Phosphorylation
Y505 Phosphorylation P41240 (CSK) , P06239 (LCK) , P42679 (MATK)

翻译修饰 - P06239 作为激酶

Substrate Site Source
O14543 (SOCS3) Y204 Uniprot
O14543 (SOCS3) Y221 Uniprot
O43561 (LAT) Y200 Uniprot
O43561 (LAT) Y220 Uniprot
P03372 (ESR1) Y537 Uniprot
P05107 (ITGB2) Y735 Uniprot
P06127 (CD5) Y453 Uniprot
P06127 (CD5) Y487 Uniprot
P06239-1 (LCK) Y394 Uniprot
P06239 (LCK) Y505 Uniprot
P07766 (CD3E) Y188 Uniprot
P07766 (CD3E) Y199 Uniprot
P10747 (CD28) Y191 Uniprot
P12235 (SLC25A4) Y195 Uniprot
P14784 (IL2RB) Y381 Uniprot
P14784 (IL2RB) Y384 Uniprot
P14784 (IL2RB) Y387 Uniprot
P14784 (IL2RB) Y418 Uniprot
P14784 (IL2RB) Y536 Uniprot
P15311 (EZR) Y146 Uniprot
P15498-1 (VAV1) Y142 Uniprot
P15498 (VAV1) Y160 Uniprot
P15498-1 (VAV1) Y174 Uniprot
P15941 (MUC1) Y1229 Uniprot
P16284 (PECAM1) Y713 Uniprot
P16410-1 (CTLA4) Y201 Uniprot
P16410 (CTLA4) Y218 Uniprot
P16885 (PLCG2) Y743 Uniprot
P16885 (PLCG2) Y753 Uniprot
P16885 (PLCG2) Y759 Uniprot
P16885 (PLCG2) Y1197 Uniprot
P16885 (PLCG2) Y1217 Uniprot
P20138 (CD33) Y340 Uniprot
P20936-2 (RASA1) Y283 Uniprot
P20936-1 (RASA1) Y460 Uniprot
P20963 (CD247) Y142 Uniprot
P22681 (CBL) Y700 Uniprot
P22681 (CBL) Y731 Uniprot
P22681 (CBL) Y774 Uniprot
P23743 (DGKA) Y335 Uniprot
P24666 (ACP1) Y132 Uniprot
P24666-1 (ACP1) Y133 Uniprot
P25963 (NFKBIA) Y42 Uniprot
P27361 (MAPK3) Y204 Uniprot
P27986-3 (PIK3R1) Y388 Uniprot
P27986-2 (PIK3R1) Y418 Uniprot
P27986-1 (PIK3R1) Y688 Uniprot
P29350 (PTPN6) Y536 Uniprot
P29350-3 (PTPN6) Y538 Uniprot
P29350-1 (PTPN6) Y564 Uniprot
P29350-3 (PTPN6) Y566 Uniprot
P29353-2 (SHC1) Y239 Uniprot
P29353-2 (SHC1) Y240 Uniprot
P29353-2 (SHC1) Y317 Uniprot
P29353-7 (SHC1) Y318 Uniprot
P29353-1 (SHC1) Y427 Uniprot
P40259-2 (CD79B) Y92 Uniprot
P40259-2 (CD79B) Y103 Uniprot
P40259-1 (CD79B) Y196 Uniprot
P40259-1 (CD79B) Y207 Uniprot
P42224-1 (STAT1) Y701 Uniprot
P42229 (STAT5A) Y694 Uniprot
P42768 (WAS) Y291 Uniprot
P43403-2 (ZAP70) Y12 Uniprot
P43403 (ZAP70) Y69 Uniprot
P43403 (ZAP70) Y126 Uniprot
P43403-2 (ZAP70) Y167 Uniprot
P43403 (ZAP70) Y178 Uniprot
P43403-2 (ZAP70) Y185 Uniprot
P43403-2 (ZAP70) Y186 Uniprot
P43403 (ZAP70) Y292 Uniprot
P43403 (ZAP70) Y315 Uniprot
P43403-1 (ZAP70) Y319 Uniprot
P43403 (ZAP70) Y474 Uniprot
P43403 (ZAP70) Y492 Uniprot
P43403 (ZAP70) Y493 Uniprot
P52630 (STAT2) Y690 Uniprot
P52735 (VAV2) Y142 Uniprot
P52735 (VAV2) Y159 Uniprot
P52735 (VAV2) Y172 Uniprot
P52757 (CHN2) Y153 Uniprot
P60484 (PTEN) Y240 Uniprot
P60484 (PTEN) Y315 Uniprot
P67775 (PPP2CA) Y307 Uniprot
Q04759 (PRKCQ) Y90 Uniprot
Q05655 (PRKCD) Y313 Uniprot
Q05655-1 (PRKCD) Y334 Uniprot
Q05655-1 (PRKCD) Y514 Uniprot
Q06830 (PRDX1) Y194 Uniprot
Q08881 (ITK) Y512 Uniprot
Q13094 (LCP2) Y113 Uniprot
Q13094 (LCP2) Y128 Uniprot
Q13094 (LCP2) Y145 Uniprot
Q13094 (LCP2) Y423 Uniprot
Q13094 (LCP2) Y426 Uniprot
Q13283 (G3BP1) Y56 Uniprot
Q13291 (SLAMF1) Y307 Uniprot
Q13444 (ADAM15) Y715 Uniprot
Q13444 (ADAM15) Y735 Uniprot
Q13813 (SPTAN1) Y1176 Uniprot
Q15831 (STK11) Y36 Uniprot
Q15831 (STK11) Y261 Uniprot
Q15831 (STK11) Y362 Uniprot
Q16539 (MAPK14) Y323 Uniprot
Q8IVM0 (CCDC50) Y217 Uniprot
Q8IVM0 (CCDC50) Y279 Uniprot
Q8IVM0 (CCDC50) Y304 Uniprot
Q8N1K5 (THEMIS) Y540 Uniprot
Q8N1K5 (THEMIS) Y541 Uniprot
Q96LC7 (SIGLEC10) Y597 Uniprot
Q96LC7 (SIGLEC10) Y667 Uniprot
Q96LC7 (SIGLEC10) Y691 Uniprot
Q99704-1 (DOK1) Y174 Uniprot
Q9BWW4 (SSBP3) Y23 Uniprot
Q9BWW4 (SSBP3) Y25 Uniprot
Q9BZS1 (FOXP3) Y342 Uniprot
Q9H204 (MED28) Y64 Uniprot
Q9H4E7 (DEF6) Y133 Uniprot
Q9H4E7 (DEF6) Y144 Uniprot
Q9H4E7 (DEF6) Y210 Uniprot
Q9NP31 (SH2D2A) Y260 Uniprot
Q9NP31 (SH2D2A) Y280 Uniprot
Q9NP31 (SH2D2A) Y290 Uniprot
Q9NP31 (SH2D2A) Y305 Uniprot
Q9UN19-1 (DAPP1) Y139 Uniprot
Q9UQQ2 (SH2B3) Y273 Uniprot

研究背景

功能:

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2.

翻译修饰:

Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling.

Myristoylation is required prior to palmitoylation.

Palmitoylation regulates subcellular location.

细胞定位:

Cytoplasm. Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Present in lipid rafts in an inactive form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed specifically in lymphoid cells.

亚基结构:

Binds to the cytoplasmic domain of cell surface receptors, such as AXL, CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB1 and to other protein kinases including CDK1, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH. Interacts with RUNX3. Forms a signaling complex with EPHA1, PTK2B AND PI3-KINASE; upon activation by EFNA1 which may regulate T-lymphocyte migration. Associates with ZAP70 and RHOH; these interactions allow LCK-mediated RHOH and CD3 subunit phosphorylation in the presence of functional ZAP70. Interacts with UNC119; this interaction plays a crucial role in activation of LCK. Interacts with CEACAM1 (via cytoplasmic domain); mediates CEACAM1 phosphorylation resulting in PTPN6 recruitment that dephosphorylates TCR stimulation-induced CD247 and ZAP70. Interacts with CD160.

(Microbial infection) Interacts with herpes simplex virus 1 UL46; this interaction activates LCK.

(Microbial infection) Interacts with HIV-1 Nef through its SH3 domain.

蛋白家族:

The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

文献引用

1). Zhang J et al. Regulation of T Cell Activities in Rheumatoid Arthritis by the Novel Fusion Protein IgD-Fc-Ig. Frontiers in Immunology 2020 May 15;11:755. (PubMed: 32499775) [IF=7.3]

Application: WB    Species: mouse    Sample: spleen

FIGURE 9 | Effects of IgD-Fc-Ig (DG) on the protein expression of Lck, p-Lck, ZAP70 and p-ZAP70. (A–C) Western blot analysis of p-Lck and p-ZAP70 expression in PBMCs of RA patients, PBMCs were treated with IgD (3µg/mL) and IgD-Fc-Ig (1, 3, 10µg/mL) for 48h and lysed. (D–F) Western blot analysis of Lck, p-Lck, ZAP70 and p-ZAP70 expression in CIA mice. Mice spleens from each group were isolated and homogenized in lysis buffer.

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