Phospho-SRPK2 (Ser494/Ser497) Antibody - #AF3633

Figure 7 Double-labeling immunofluorescence and confocal microscopy to p38-P Thr180/Tyr182 or SAPK/JNK-PThr183/Thr185 (green), and AT8 (red) in inoculated hTau at the age of six months and killed at the age of nine months shows co-localization of the active kinases with phospho-tau deposition (white arrows) (A and B, respectively for p38-P and SAPK-JNK-P) in dentate gyrus neurons. Double-labelling immunofluorescence and confocal microscopy to PKAα/β-P Tyr197 and AT8 in inoculated hTau mice shows cytoplasmic granular PKAα/β-P Tyr197 granules in CA1 neurons with abnormal phospho-tau deposits in neurons (C, arrow). Cytoplasmic SRPK2-Ser497 immunoreactivity is found in neurons with phospho-tau deposits in inoculated hTau mice (D). Paraffin sections, nuclei are stained with DRAQ5™ (blue), A, B, bar = 10 µm, C and D, bar = 20 µm. CLK1 immunoreactivity in the CA1 region of non-inoculated (E) and inoculated hTau with AD sarkosyl-insoluble fractions (F); small cytoplasmic granules are seen in inoculated hTau. Paraffin sections, slightly counterstained with haematoxylin, bar = 40 µm.