Synaptophysin Antibody - #AF0257

Figure 3. The effect of Sig-1R agonist on synapse change in the hippocampus of mice with T1DM. (a–c) Representative Western blots and quantitation of PSD-95 and Syp protein expression in CON, CON + PRE-084, STZ, and STZ + PRE-084 mice (n = 6). (d–g) Representative images of synaptic ultrastructure (d), quantitative analysis of PSD length (e) and width (f), and synaptic cleft (g) (n = 85–90; 3 animals per group). The scale bars are 500 and 200 nm. (h) Golgi staining images of the neuronal morphology. The scale bar is 50 μm. (i) Representative three-dimensional reconstruction images of different types of the spine in hippocampal neurons, including stubby (red), mushroom (green), thin (blue), and filopodia (purple) spines. The scale bar is 2 μm. (j–n) Quantitation of density of different spines, including total (j), stubby (k), mushroom (l), thin (m), and filopodia (n) spines (n = 20; 3 mice per group). The data were presented as mean ± SEM and analyzed via one-way ANOVA with Tukey’s multiple comparison tests, Welch’s ANOVA test followed by Dunnett’s T3 multiple comparison tests, and Kruskal-Wallis test followed by Dunn’s multiple comparison tests. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Sig-1R, sigma-1 receptor; T1DM, type 1 diabetes mellitus; PSD, postsynaptic density; Syp, synaptophysin; CON, control mice; STZ, mice with T1DM.

Figure 5 Western blot analysis. The expressions of synaptic plasticity proteins were determined by western blot (A), including PSD-95 (B), GAP-43 (C), and SYN (D). The values represent the mean ± SEM, n = 5. *p < 0.05, **p < 0.01 vs. CON, #p < 0.05, ##p < 0.01 vs. MS. CON, control; MS, maternal separation; CUMS, chronic unpredictable mild stress.