Estrogen Receptor-alpha Antibody - #AF6058

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产品: Estrogen Receptor-alpha 抗体
货号: AF6058
描述: Rabbit polyclonal antibody to Estrogen Receptor-alpha
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
蛋白号: P03372
RRID: AB_2834976

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Estrogen Receptor-alpha Antibody detects endogenous levels of total Estrogen Receptor-alpha.
RRID:
AB_2834976
引用格式: Affinity Biosciences Cat# AF6058, RRID:AB_2834976.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp686N23123; ER alpha; ER; ER-alpha; Era; ESR; ESR1; ESR1_HUMAN; ESRA; Estradiol receptor; Estrogen nuclear receptor alpha; Estrogen receptor 1; Estrogen receptor alpha 3*,4,5,6,7*/822 isoform; Estrogen receptor alpha; Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform; Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform; Estrogen receptor alpha delta 4 +49 isoform; Estrogen receptor alpha delta 4*,5,6,7*/654 isoform; Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform; Estrogen receptor alpha E1 E2 1 2; Estrogen receptor alpha E1 N2 E2 1 2; Estrogen receptor; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human Estrogen Receptor-alpha, corresponding to a region within C-terminal amino acids.

基因/基因ID:
ESR1(2099)
描述:
ER-alpha is a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

文献引用

1). Estrogen and glucocorticoid promote the lactoferrin synthesis and secretion ability of bovine mammary epithelial cells through ER and GR signaling pathways. International journal of biological macromolecules, 2025 (PubMed: 39904446) [IF=7.7]
2). Extract of Curculigo capitulata Ameliorates Postmenopausal Osteoporosis by Promoting Osteoblast Proliferation and Differentiation. Cells, 2024 (PubMed: 39682775) [IF=6.0]
3). Crocetin Alleviates Ovariectomy-Induced Metabolic Dysfunction through Regulating Estrogen Receptor β. Journal of agricultural and food chemistry, 2021 (PubMed: 34851635) [IF=5.7]
4). Mechanism of Astragalus membranaceus (Huangqi, HQ) for treatment of heart failure based on network pharmacology and molecular docking. Journal of cellular and molecular medicine, 2024 (PubMed: 38780500) [IF=5.3]

Application: WB    Species: Human    Sample:

FIGURE 7 Effects of five active compounds on ESR1 level in Ang II‐induced cardiomyocytes. Cardiomyocytes were treated with Ang II (1 μM) for 24 h in the presence or absence of Quercetin (20 μM), Isorhamnetin (50 μM), Calycosin (20 μM), Kaempferol (20 μM), or Formononetin (20 μM) and then examined for the ESR1 level in cardiomyocytes by ELISA assay (A); the protein levels of ESR1 and phosphorylated‐ESR1 in cardiomyocytes by western blot assay (B). N = 3 (biological replicates). ***p 
5). Alleviation of Fufang Fanshiliu decoction on type II diabetes mellitus by reducing insulin resistance: A comprehensive network prediction and experimental validation. Journal of Ethnopharmacology, 2022 (PubMed: 35568115) [IF=4.8]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 8. FFSLD alleviated IR in the HepG2-IR cells model by regulating the PI3K-AKT and ESR pathways. (A, C) Western Blot detection and (B, D) quantitative analysis for the expressions of p-AKT, ESR1 in HepG2-IR cells treated with different concentrations of FFSLD for 24 h. Data (n = 3) are expressed as mean ± SEM. *p < 0.05, comparing with control.
6). Bu-shen-zhu-yun decoction inhibits granulosa cell apoptosis in rat polycystic ovary syndrome through estrogen receptor α-mediated PI3K/AKT/mTOR pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 34861362) [IF=4.8]
7). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=4.2]
8). The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice. Frontiers in Endocrinology, 2021 (PubMed: 34925230) [IF=3.9]

Application: WB    Species: Mice    Sample: femoral and spine

Figure 5 The expression level of estrogen receptors (ERs) in femur and spine. (A) Western blotting results of expression level of ERα and ERβ in femoral and spine growth plate (GP), n = 3. (B–E) Semiquantitative analysis of ER expression levels. (F) Quantitative real-time PCR results of ER genes in femoral and vertebral GP, n = 4. (G–J) Immunohistochemistry (IHC) results of expression level of ERα and ERβ in femoral and vertebral GP, n = 6. Scale bar: 20 μm. (K) Comparison of ERα HSCORE between femoral and spine GP (FGP, femoral growth plate; SGP, spine growth plate). (L, M) Results of Spearman’s analysis between ERα HSCORE and femur/spine length. *p < 0.05, ***p < 0.001 and ****p < 0.0001; ns denotes not significant.

Application: IHC    Species: Mice    Sample: femoral and vertebral

Figure 5 The expression level of estrogen receptors (ERs) in femur and spine. (A) Western blotting results of expression level of ERα and ERβ in femoral and spine growth plate (GP), n = 3. (B–E) Semiquantitative analysis of ER expression levels. (F) Quantitative real-time PCR results of ER genes in femoral and vertebral GP, n = 4. (G–J) Immunohistochemistry (IHC) results of expression level of ERα and ERβ in femoral and vertebral GP, n = 6. Scale bar: 20 μm. (K) Comparison of ERα HSCORE between femoral and spine GP (FGP, femoral growth plate; SGP, spine growth plate). (L, M) Results of Spearman’s analysis between ERα HSCORE and femur/spine length. *p < 0.05, ***p < 0.001 and ****p < 0.0001; ns denotes not significant.
9). Estrogen enhances the proliferation, migration, and invasion of papillary thyroid carcinoma via the ERα/KRT19 signaling axis. Journal of endocrinological investigation, 2025 (PubMed: 39453570) [IF=3.9]
10). Estrogen receptors and hypoxia inducible factor 1 alpha expression in abdominal wall endometriosis. REPRODUCTIVE BIOMEDICINE ONLINE, 2020 (PubMed: 32444257) [IF=3.7]

Application: IHC    Species: human    Sample: endometrium

FIGURE 1| Expression and localization of oestrogen receptors and hypoxia-inducible factor-1alpha (HIF-1a) proteins in proliferative phase and secretary phase of normal control endometrium analysed by immunohistochemistry. (A–D) ERa; (E–H) ERb; (I–L) G protein-coupled oestrogen receptor (GPER); (M–P) HIF-1a. Micrographs were taken at magnifications of × 200 (left panels) and marked areas in left-hand panels shown at × 400 (right panels) respectively. ER, oestrogen receptor.
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