Lamin A/C Antibody - #AF6056

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产品: Lamin A/C 抗体
货号: AF6056
描述: Rabbit polyclonal antibody to Lamin A/C
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Chicken, Xenopus
蛋白号: P02545
RRID: AB_2834975

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Lamin A/C Antibody detects endogenous levels of total Lamin A/C.
RRID:
AB_2834975
引用格式: Affinity Biosciences Cat# AF6056, RRID:AB_2834975.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

70 kDa lamin; Cardiomyopathy dilated 1A (autosomal dominant); CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; Lamin A; Lamin A/C; Lamin A/C like 1; Lamin; Lamin C; Lamin-A/C; LDP1; LFP; LGMD1B; Limb girdle muscular dystrophy 1B (autosomal dominant); LMN 1; LMN A; LMN C; LMN1; LMNA; LMNA_HUMAN; LMNC; LMNL1; Prelamin A/C; PRO1; Renal carcinoma antigen NY REN 32; Renal carcinoma antigen NY-REN-32; Renal carcinoma antigen NYREN32;

抗原和靶标

免疫原:

A synthesized peptide derived from human Lamin A/C, corresponding to a region within the internal amino acids.

基因/基因ID:
LMNA(4000)
描述:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

文献引用

1). Metabolic regulation of homologous recombination repair by MRE11 lactylation. Cell, 2024 (PubMed: 38128537) [IF=45.5]

Application: WB    Species: Human    Sample: MDA-MB-231 cells

Figure S3. MRE11 lactylation enhances its chromatin loading, DNA binding, and DNA end resection, related to Figures 3 and 4. (F–H) Western blots of soluble fractions from the indicated cells. These figures are related to Figures 3I, 3J, and 3L.
2). Phosphoproteomic analysis reveals the mechanisms of human umbilical cord mesenchymal stem cell-derived exosomes attenuate renal aging. Journal of proteomics, 2025 (PubMed: 39433154) [IF=2.8]

Application: WB    Species: Mouse    Sample:

Fig. 7. Western blotting and immunohistochemical staining validation of selected differentially phosphorylated proteins. A. Proteins expression of Lamin A/C, phosphorylated Lamin A/C(Ser390) and phosphorylated Lamin A/C(Ser392) in the kidneys of WT-AC and WT-AEX mice were detected by western blotting and quantified by densitometry. B. Immunochemistry staining of Lamin A/C in kidney tissues (scale bar = 20 μm). C. Immunochemistry staining of Phospho-Lamin A/C(Ser390) in kidney tissues (scale bar = 20 μm). D. Immunochemistry staining of Phospho-Lamin A/C(Ser392) in kidney tissues (scale bar = 20 μm). Data are presented as the mean ± SEM. Not significant (ns) p>0.05, ⁎p < 0.05, ⁎⁎p < 0.01, ⁎⁎⁎p < 0.001.

Application: IHC    Species: Mouse    Sample:

Fig. 7. Western blotting and immunohistochemical staining validation of selected differentially phosphorylated proteins. A. Proteins expression of Lamin A/C, phosphorylated Lamin A/C(Ser390) and phosphorylated Lamin A/C(Ser392) in the kidneys of WT-AC and WT-AEX mice were detected by western blotting and quantified by densitometry. B. Immunochemistry staining of Lamin A/C in kidney tissues (scale bar = 20 μm). C. Immunochemistry staining of Phospho-Lamin A/C(Ser390) in kidney tissues (scale bar = 20 μm). D. Immunochemistry staining of Phospho-Lamin A/C(Ser392) in kidney tissues (scale bar = 20 μm). Data are presented as the mean ± SEM. Not significant (ns) p>0.05, ⁎p < 0.05, ⁎⁎p < 0.01, ⁎⁎⁎p < 0.001.
3). Exosomes Derived from BMSCs Ameliorate Intestinal Ischemia-Reperfusion Injury by Regulating miR-144-3p-Mediated Oxidative Stress. Digestive diseases and sciences, 2022 (PubMed: 35624329) [IF=2.5]

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