Phospho-SPHK2 (Ser387) Antibody - #AF3532

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产品: 磷酸化 SPHK2 (Ser387) 抗体
货号: AF3532
描述: Rabbit polyclonal antibody to Phospho-SPHK2 (Ser387)
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: Q9NRA0
RRID: AB_2846846

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-SPHK2 (Ser387) Antibody detects endogenous levels of SPHK2 only when phosphorylated at Ser387.
RRID:
AB_2846846
引用格式: Affinity Biosciences Cat# AF3532, RRID:AB_2846846.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

SK 2; Sphingosine kinase 2; Sphingosine kinase type 2; SPHK2; SPHK2_HUMAN; SPK 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human SPHK2 around the phosphorylation site of Ser387.

基因/基因ID:
SPHK2(56848)

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

文献引用

1). Dysregulation of sphingolipid metabolism contributes to the pathogenesis of chronic myeloid leukemia. Cell death & disease, 2025 (PubMed: 40221405) [IF=8.1]
2). Nuclear SPHK2/S1P induces oxidative stress and NLRP3 inflammasome activation via promoting p53 acetylation in lipopolysaccharide-induced acute lung injury. Cell Death Discovery, 2023 (PubMed: 36653338) [IF=7.0]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig. 2 Knockdown SPHK2 restrains LPS-induced M1 macrophage polarization and inflammation. A RAW264.7 cells were stimulated with LPS (1 μg/mL) for 0 (NC), 6, 12, or 24 h. Western blot analysis of NLRP3, ASC and Caspase-1 p20 protein expression levels in LPS-treated RAW264.7 cells. B The levels of SPHK2 and C p-SPHK2 protein were analyzed by immunoblotting. D Representative immunofluorescent images of CD206 or iNOS (red) in LPS-treated RAW264.7 macrophages transfected with sh-SPHK2 or shNC. bar = 50 μm. E Knockdown SPHK2 could significantly decrease the production of LPS-induced inflammatory cytokines (IL-1β, TNF-α, iNOS, COX-2, and IL-6) in macrophages. Data were presented as the means ± SEM from at least three independent experiments. *p 

Application: IF/ICC    Species: Mouse    Sample: RAW264.7 cells

Fig. 2 Knockdown SPHK2 restrains LPS-induced M1 macrophage polarization and inflammation. A RAW264.7 cells were stimulated with LPS (1 μg/mL) for 0 (NC), 6, 12, or 24 h. Western blot analysis of NLRP3, ASC and Caspase-1 p20 protein expression levels in LPS-treated RAW264.7 cells. B The levels of SPHK2 and C p-SPHK2 protein were analyzed by immunoblotting. D Representative immunofluorescent images of CD206 or iNOS (red) in LPS-treated RAW264.7 macrophages transfected with sh-SPHK2 or shNC. bar = 50 μm. E Knockdown SPHK2 could significantly decrease the production of LPS-induced inflammatory cytokines (IL-1β, TNF-α, iNOS, COX-2, and IL-6) in macrophages. Data were presented as the means ± SEM from at least three independent experiments. *p 

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