Bcl-2 Antibody - #AF6138

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产品: Bcl-2 抗体
货号: AF6138
描述: Rabbit polyclonal antibody to Bcl-2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P10415
RRID: AB_2846805

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Bcl-2 Antibody detects endogenous levels of total Bcl-2.
RRID:
AB_2846805
引用格式: Affinity Biosciences Cat# AF6138, RRID:AB_2846805.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptosis regulator Bcl 2; Apoptosis regulator Bcl-2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; Bcl-2; Bcl2; BCL2_HUMAN; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; PPP1R50; Protein phosphatase 1, regulatory subunit 50;

抗原和靶标

免疫原:

A synthesized peptide derived from human Bcl-2.

基因/基因ID:
BCL2(596)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury. Advanced Healthcare Materials, 2023 (PubMed: 37097199) [IF=10.0]
2). A novel nuclear receptor NR1D1 suppresses HSD17B12 transcription to regulate granulosa cell apoptosis and autophagy via the AMPK pathway in sheep. International journal of biological macromolecules, 2025 (PubMed: 39986531) [IF=7.7]
3). The reduced SCFA-producing gut microbes are involved in the inflammatory activation in Kawasaki disease. Frontiers in Immunology, 2023 (PubMed: 37398673) [IF=5.7]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Figure 6 Butyrate promoted MPK-1 expression to inhibit the MAPK signaling pathways in CAWS-stimulated RAW264.7. (A) The mRNA expression level of IL-1β, IL-6, TNF-α and MCP-1 was examined in RAW264.7 cells treated with SP600125 (JNK inhibitor, 5 μmol/L), SCH772984 (ERK1/2 inhibitor, 300 nmol/L) and SB203580 (p38 inhibitor, 5 μmol/L), respectively. (B) The expression of phosphorylation of JNK, p38 and ERK was detected after butyrate treatment. (C) The mRNA expression of phosphatases MKP-1, PP1, PP2, PP2A, PTP1B and SHP2 in RAW264.7 after butyrate treatment. (D) The expression of MKP-1 was evaluated by using western blot. (E) The effect of MKP-1 knockdown was assessed by analyzing the phosphorylation level of JNK, p38 and ERK and expression of IL-6 and TNF-α. Values are expressed as the mean ± SEM. Significance: * P < 0.05, ** P < 0.01, *** P < 0.001, vs. the control group; # P < 0.05, ## P < 0.01, ### P < 0.01 vs. the CAWS group; ns means no significance.
4). Hypoxia Regulates the Proliferation and Apoptosis of Coronary Artery Smooth Muscle Cells Through HIF-1α Mediated Autophagy in Yak. Biomolecules, 2025 (PubMed: 40001559) [IF=5.5]

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