c Abl Antibody - #AF6038

Fig. 6 C-Abl expression is regulated by the Hippo/YAP signaling pathway in cochlear HCs after neomycin exposure. A The cochleae were dissected from P3 WT mice, and immunolabeled with Myosin7a (green), Sox2 (blue), and C-Abl (red). Immunofluorescence staining showed the expression and localization of C-Abl in the P3 WT mouse cochlea under a 63 × microscope, and C-Abl was expressed in the nuclei of cochlear HCs. B Schematic diagram of drug addition in tissue culture (divided into three groups). C Immunofluorescence staining with C-Abl and Myosin7a in the middle turn of the cochlear basilar membrane after different treatments. The expression of C-Abl was significantly increased in the neomycin-treated group and decreased in the XMU/neomycin-treated group. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3. Scale bars = 20 µm

Fig. 7 YAP Overexpression inhibits C-Abl-mediated HC apoptosis in cochlear HCs after neomycin damage. A Schematic diagram of drug addition in tissue culture (divided into four groups). B The cochleae were dissected from P3 WT mice used for Western blot experiment. Western blot showed that the expression of C-Abl was significantly increased in the neomycin-treated group and decreased in the XMU/neomycin-treated group. C, D The mRNA levels of C-Abl signaling downstream genes were analyzed by qPCR in the different treatment groups. The qPCR results showed that XMU downregulated the expression of C-Abl and p73 and that VP upregulated the expression of C-Abl and p73. *p < 0.05, **p < 0.01, ***p < 0.001, n = 3. Scale bars = 20 µm

Fig. 3. Mechanisms underlying the effects of TET on the chemosensitivity of PANC-1/GEM cells to GEM. (A) Detection of Hoechst 33258-stained nuclei in PANC-1/ GEM cells treated as indicated. (B) Detection of apoptosis in PANC-1/GEM cells treated as indicated. PANC-1/GEM cells were treated with 2 μ g/mL GEM, 40 μ g/mL TET, or 5 μ M MHY1485 for 24 h. (C) Expression of PI3K/Akt/mTOR signaling-related proteins in cells treated as indicated and analyzed by immunoblotting. GAPDH was used as the loading control. Representative images of the expression profiles of all protein from three independent experiments are shown. (D) Expression profile of survivin gene in PANC-1/GEM cells treated with GEM and TET, alone or in combination. After treatment with various concentrations of GEM (0, 1, and 2 μ g/mL) and TET (0, 20, and 40 μ g/mL), alone or in combination, for 48 h, the expression profile of the survivin gene was analyzed using real-time PCR. ***P < 0.001.