(184)
(63)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GSDMD Antibody detects endogenous levels of total GSDMD protein, including precursor and the cleaved N-terminal fragment.
RRID:
AB_2846776
引用格式: Affinity Biosciences Cat# AF4012, RRID:AB_2846776.
引用格式: Affinity Biosciences Cat# AF4012, RRID:AB_2846776.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
1810036L03Rik; DF 5L; DF5L; DFNA 5L; DFNA5L; FKSG 10; FKSG10; FLJ12150; Gasdermin D; Gasdermin domain containing 1; Gasdermin domain containing protein 1; Gasdermin domain-containing protein 1; Gasdermin-D; GasderminD; GSDMD; GSDMD_HUMAN; GSDMDC 1; GSDMDC1;
抗原和靶标
研究领域
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
文献引用
1). Stem cell-derived exosomes prevent the development of thoracic aortic aneurysm/dissection by inhibiting AIM2 inflammasome and pyroptosis. Extracellular Vesicle, 2024
[IF=16.0]
2). Cascade-Type Microglial Pyroptosis Inhibitors for Enhanced Treatment of Cerebral Ischemia-Reperfusion Injury. ACS nano, 2025
(PubMed: 40047143)
[IF=15.8]
3). Single-Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024
(PubMed: 38036420)
[IF=15.1]
Application: IHC Species: Human Sample:
Figure 5 Pyroptotic macrophages promote stromal cell senescence. A) Representative images of senescence‐associated β‐galactosidase (SA‐beta‐gal) staining of stromal cells treated with the supernatant of TNF‐α/IFN‐γ‐stimulated THP1 cells, the supernatant of LPS/Nig‐stimulated THP1 cells or the supernatant of unstimulated THP1 cells (scale bar, 500 µm). Statistical analyses of SA‐beta‐gal‐positive cell rates were shown in the right image. B) The relative expression of fibrosis‐related genes in ovarian stromal cells treated with supernatant of unstimulated or stimulated THP1 cells. The results were analyzed by ANOVA. C) Representative IHC staining for GSDMD showing the elevated expression of GSDMD in the middle‐aged group (scale bar, 100 µm). The insert at top left corner of each image is the magnified image (scale bar, 250 µm). D) EREG concentration in follicular fluids measured by ELISA. E) Flow cytometric analysis of follicular fluids from IVF patients: representative flow cytometric dot plots of macrophages in follicular fluids from each group. Statistical graph of the percentages of macrophages in follicular fluids. F) Representative histogram plots and the statistical graph of the MFI of GSDMD. MFI: mean fluorescence intensity.
Application: IF/ICC Species: Human Sample:
Figure 6 The inhibition of pyroptosis impedes ovarian aging and promotes female health. A) The expression levels of pyroptosis‐related proteins in ovaries from naturally aged (old), prematurely aged (POI) and LPS‐stimulated mice were detected by western blotting. B) Representative immunofluorescence images of GSDMD staining of ovarian sections from young and old mice, with nuclei counterstained with DAPI (scale bar, 50 µm). C) Representative HE‐stained ovarian tissue sections from mice of different genotypes at the ages of 3 months (3 mo), 8 months (8 mo), and 13 months (13 mo). D) The total number of follicles of each stage was counted and statistically analyzed. The error bars indicate SD. *p
4). Gut microbes exacerbate systemic inflammation and behavior disorders in neurologic disease CADASIL. Microbiome, 2023
(PubMed: 37684694)
[IF=13.8]
5). Inhibition of macrophage inflammasome assembly and pyroptosis with GC-1 ameliorates acute lung injury. Theranostics, 2025
(PubMed: 39990234)
[IF=12.4]
6). Exosomal miRNA reprogramming in pyroptotic macrophage drives silica-induced fibroblast-to-myofibroblast transition and pulmonary fibrosis. Journal of hazardous materials, 2024
(PubMed: 39603130)
[IF=12.2]
7). Force-induced Caspase-1-dependent pyroptosis regulates orthodontic tooth movement. International journal of oral science, 2024
(PubMed: 38221531)
[IF=10.8]
8). Hair follicle-MSC-derived small extracellular vesicles as a novel remedy for acute pancreatitis. JOURNAL OF CONTROLLED RELEASE, 2022
(PubMed: 36402231)
[IF=10.5]
9). WTAP-mediated N6-methyladenosine modification of NLRP3 mRNA in kidney injury of diabetic nephropathy. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2022
(PubMed: 35761192)
[IF=9.2]
Application: WB Species: Human Sample: HK-2 cells
"Fig. 2
Knockdown of WTAP inhibits high glucose (HG)-induced cell pyroptosis and pro-inflammatory factor release in HK-2 cells. A Cell viability; (B, C) cell pyroptosis; (D) LDH activity; E, F expression of WTAP, NLRP3, pro-caspase-1, active caspase-1, GSDMD, and GSDMD-N; and (G, H) the release of IL-18, IL-1β, TNF-α, and IL-6 in HK-2 cells treated with NG or HG with or without WTAP knockdown (n = 3). One-way analysis of variance was used to analyze the data among multiple groups, followed by Tukey’s post hoc test. ***P < 0.001 compared with NG + shNC. ##P < 0.01, ###P < 0.001 compared with HG + shNC"
10). Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancer. Journal of biomedical science, 2024
(PubMed: 38862973)
[IF=9.0]
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.