SPC25 Antibody - #DF13747

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产品: SPC25 抗体
货号: DF13747
描述: Rabbit polyclonal antibody to SPC25
应用: WB
文献验证: WB
反应: Human
预测: Bovine, Horse, Rabbit, Dog
蛋白号: Q9HBM1
RRID: AB_2846766

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
SPC25 Antibody detects endogenous levels of total SPC25.
RRID:
AB_2846766
引用格式: Affinity Biosciences Cat# DF13747, RRID:AB_2846766.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25; SPC25_HUMAN; spindle pole body component 25 homolog;

抗原和靶标

免疫原:

A synthesized peptide derived from human SPC25, corresponding to a region within N-terminal amino acids.

基因/基因ID:
SPC25(57405)

文献引用

1). SPC25 as a novel therapeutic and prognostic biomarker and its association with glycolysis, ferroptosis and ceRNA in lung adenocarcinoma. Aging (Albany NY), 2024 (PubMed: 38217547)

Application: WB    Species: human    Sample: H1975 cells

Figure 3. The impact of knocking down SPC25 on LUAD. (A, B) An analysis of Western blotting confirmed the success of SPC25-siRNAs transfection into H1975 cells and their ability to interfere with gene expression. (C, D) CCK-8 assay evaluated tumor cell proliferation activity. (E, F) EdU staining of H1975 cells and EdU-positive cell proportion. (G, H) The effect of SPC25 knockdown on apoptosis of the H1975 cells as detected by flow cytometry. (I–K) Representative images and quantitative analysis of wound healing measurements in LUAD cells. (***p < 0.001; **p < 0.01; *p < 0.05; Abbreviation: ns: not significant).

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