产品: TBX5 抗体
货号: DF13705
描述: Rabbit polyclonal antibody to TBX5
应用: WB
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q99593
RRID: AB_2846724

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TBX5 Antibody detects endogenous levels of total TBX5.
RRID:
AB_2846724
引用格式: Affinity Biosciences Cat# DF13705, RRID:AB_2846724.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Holt Oram syndrome; HOS; T box 5; T box protein 5; T box transcription factor TBX 5; T box transcription factor TBX5; T-box protein 5; T-box transcription factor TBX5; TBX 5; TBX5; TBX5_HUMAN; Transcription factor T box 5;

抗原和靶标

免疫原:

A synthesized peptide derived from human TBX5, corresponding to a region within the internal amino acids.

基因/基因ID:

文献引用

1). Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 editing. Stem Cell Research, 2021 (PubMed: 33497883) [IF=0.8]

Application: WB    Species: Human    Sample: hESC H9

Since TBX5 is mainly expressed in the heart, we verified the deletion of TBX5 by Western blot at the protein level (Fig. 1I). The results showed that TBX5 was completely knocked out in TBX5-KO cardiomyocytes (Fig. 1). Short tandem repeat (STR) analysis showed that TBX5-KO is the same as H9. Table 1 summarizes the characterization of TBX5-KO.

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