UNC5B Antibody - #DF13685

Figure 6 UNC5B receptor is a key in reprogramming generated by PTBP1 knockdown. (A-C) KI67 and TUJ1 staining revealed the reprogramming efficiency of particular lentiviruses-infected U251 cells (9 random fields from triplicate samples were captured for quantification; TUJ1+ (%) = TUJ1+ M-cherry+/M-cherry+; KI67+ (%) = KI67+ M-cherry+/M-cherry+; M-cherry+ cells = 128-566 for each condition). (D, E) Western blot analysis of UNC5B protein in U251 cells infected with sh-Luci and sh-PTBP1 for 3 and 7 days. (n = 3). (F, G) The change of UNC5B protein expression under the state of PTBP1 overexpression. (n = 3). GAPDH was used as an internal reference protein. The data are presented as mean ± SD. ***P < 0.001, ###P < 0.001 vs. sh-PTBP1, sh-Luci, sh-Luci-3d or control vector group. Dpi (d): days post infection; ND: not detected; NS (ns): no significance; OE: overexpression. Scale: 100 µm.

FIGURE 2 KOA synovial fibrosis initiates Netrin-1 induction of sensory nerve germination. (A) Each group of DRG tissues was immunofluorescently stained using βIII-Tubulin + DCC + DAPI at 200× scale bar = 50 μm to observe the expression of DRG markers and DCC, an indicator of nerve sprouting. (B) Each group of DRG tissues was immunofluorescently stained using βIII-Tubulin + UNC5 + DAPI, 200× scale bar = 50 μm, to observe the expression of DRG markers and UNC5, an indicator of nerve sprouting. (C–E) WB and PCR were performed to detect the protein and gene expression of GAP43, DCC and UNC5, the neural sprouting-related indicators in DRG tissues of each group of rats. (*p 

FIGURE 2 KOA synovial fibrosis initiates Netrin-1 induction of sensory nerve germination. (A) Each group of DRG tissues was immunofluorescently stained using βIII-Tubulin + DCC + DAPI at 200× scale bar = 50 μm to observe the expression of DRG markers and DCC, an indicator of nerve sprouting. (B) Each group of DRG tissues was immunofluorescently stained using βIII-Tubulin + UNC5 + DAPI, 200× scale bar = 50 μm, to observe the expression of DRG markers and UNC5, an indicator of nerve sprouting. (C–E) WB and PCR were performed to detect the protein and gene expression of GAP43, DCC and UNC5, the neural sprouting-related indicators in DRG tissues of each group of rats. (*p