| 产品: | VPS36 抗体 |
| 货号: | DF13682 |
| 描述: | Rabbit polyclonal antibody to VPS36 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 43kDa; 44kD(Calculated). |
| 蛋白号: | Q86VN1 |
| RRID: | AB_2846701 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13682, RRID:AB_2846701.
展开/折叠
C13orf9; CGI 145; EAP45; ELL associated protein of 45 kDa; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36; Vacuolar protein sorting associated protein 36; Vacuolar protein-sorting-associated protein 36; Vps36; VPS36_HUMAN;
抗原和靶标
- Q86VN1 VPS36_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86VN1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot |
研究背景
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
Cytoplasm. Endosome. Late endosome. Membrane. Nucleus.
Note: Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.
Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25. Interacts with VPS25, SNF8, TSG101 and CHMP6. Interacts (via GLUE domain) with ubiquitin. Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with ECPAS.
The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
Belongs to the VPS36 family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.