MITF Antibody - #AF6027

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产品: MITF 抗体
货号: AF6027
描述: Rabbit polyclonal antibody to MITF
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Monkey
预测: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
蛋白号: O75030
RRID: AB_2834960

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Monkey
克隆:
Polyclonal
特异性:
MITF Antibody detects endogenous levels of total MITF.
RRID:
AB_2834960
引用格式: Affinity Biosciences Cat# AF6027, RRID:AB_2834960.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BHLHE32; bHLHe32; Class E basic helix-loop-helix protein 32; CMM8; Homolog of mouse microphthalmia; Mi; Microphthalmia associated transcription factor; Microphthalmia, mouse, homolog of; Microphthalmia-associated transcription factor; MITF; MITF_HUMAN; mitfa; nacre; WS2; WS2A; z3A.1;

抗原和靶标

免疫原:

A synthesized peptide derived from human MITF, corresponding to a region within the internal amino acids.

基因/基因ID:
MITF(4286)
描述:
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

文献引用

1). The critical role of ROS in Ermanin-induced melanogenesis. Free radical biology & medicine, 2021 (PubMed: 34560247) [IF=7.1]
2). Galangin ameliorates PTU-induced vitiligo in zebrafish and B16F10 cells by increasing melanogenesis through activation of the p38/JNK MAPK pathway. Frontiers in pharmacology, 2025 (PubMed: 40129935) [IF=5.6]

Application: WB    Species: Mouse    Sample: B16F10 cells

Figure 8. Effect of GA on the expression of key signaling pathway proteins. (A) Western blotting to detect the expression levels of p38 MAPK, ERK and JNK. (B–D) Effect of GA on the expression of p38, JNK1/2/3 and ERK1/2 proteins in B16F10 cells (n = 3). (E) Western blotting to detect the expression levels of MITF, TYR, TRP2 and TRP1. (F–I) Effect of GA on MITF, TYR, TRP2 and TRP1 protein expression in B16F10 cells (n = 3).
3). Fast freezing inhibits melanin synthesis of melanocytes by modulating the Wnt/β-catenin signalling pathway. Experimental dermatology, 2024 (PubMed: 38770555) [IF=3.5]

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