产品: Glucose 6 phosphate isomerase 抗体
货号: DF13660
描述: Rabbit polyclonal antibody to Glucose 6 phosphate isomerase
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 63kDa; 63kD(Calculated).
蛋白号: P06744
RRID: AB_2846679

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Glucose 6 phosphate isomerase Antibody detects endogenous levels of total Glucose 6 phosphate isomerase.
RRID:
AB_2846679
引用格式: Affinity Biosciences Cat# DF13660, RRID:AB_2846679.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PGI; PHI; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; Phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P06744 作为底物

Site PTM Type Enzyme
A2 Acetylation
K12 Acetylation
K12 Methylation
K12 Ubiquitination
Y17 Phosphorylation
S22 Phosphorylation
K34 Acetylation
K34 Ubiquitination
Y55 Phosphorylation
S56 Phosphorylation
T61 Phosphorylation
R66 Methylation
K73 Ubiquitination
S74 Phosphorylation
K89 Acetylation
K89 Ubiquitination
Y92 Phosphorylation
T93 Phosphorylation
R106 Methylation
S107 Phosphorylation
T109 Phosphorylation
K116 Acetylation
K116 Ubiquitination
K124 Acetylation
K124 Ubiquitination
K130 Ubiquitination
S131 Phosphorylation
S138 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K147 Ubiquitination
S160 Phosphorylation
T168 Phosphorylation
K172 Ubiquitination
Y174 Phosphorylation
S176 Phosphorylation
Y183 Phosphorylation
S185 Phosphorylation P68400 (CSNK2A1)
K194 Ubiquitination
T195 Phosphorylation
S210 Phosphorylation
K211 Acetylation
K211 Ubiquitination
T214 Phosphorylation
T215 Phosphorylation
K226 Ubiquitination
K234 Acetylation
K234 Ubiquitination
S237 Phosphorylation
K241 Ubiquitination
S247 Phosphorylation
T248 Phosphorylation
T250 Phosphorylation
T251 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S359 Phosphorylation
K362 Acetylation
K362 Ubiquitination
K366 Ubiquitination
C404 S-Nitrosylation
T412 Phosphorylation
K423 Ubiquitination
R438 Methylation
S441 Phosphorylation
K447 Ubiquitination
K454 Acetylation
K454 Ubiquitination
S455 Phosphorylation
K466 Acetylation
K466 Sumoylation
K466 Ubiquitination
K524 Ubiquitination
S532 Phosphorylation

研究背景

功能:

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.

翻译修饰:

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

ISGylated.

细胞定位:

Cytoplasm. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer; in the catalytically active form. Monomer in the secreted form.

蛋白家族:

Belongs to the GPI family.

研究领域

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

文献引用

1). Novel long non‐coding RNA CYB561‐5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non‐small cell lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 35064752) [IF=5.3]

Application: WB    Species: Human    Sample: H1299 cells

FIGURE 4 Lnc‐CYB561‐5 promotes aerobic glycolysis in vivo. (A) Cluster analysis of differentially expressed mRNAs in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (B) The top 20 GO enrichment of significantly upregulated genes (fold change > 2, p < 0.05, top 100). (C) Heatmap depicting relative expression of known genes related to glycolysis. (D and E) Measurement of ECARO and CR in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (F) Relative glycolysis rates in H1299 cells, as judged by Seahorse analyses. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. *p < 0.05, **p < 0.01, ***p < 0.001, NS, p > 0.05 vs. the sh‐Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one‐way ANOVA followed by Tukey's post hoc test

Application: WB    Species: human    Sample: H1299 cells

FIGURE 4| Lnc-CYB561-5 promotes aerobic glycolysis in vivo. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. *p < 0.05, **p < 0.01, ***p < 0.001,NS, p > 0.05 vs. the sh-Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one-way ANOVA followed by Tukey's post hoc test

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