UBR4 Antibody - #DF13659

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产品: UBR4 抗体
货号: DF13659
描述: Rabbit polyclonal antibody to UBR4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 573kDa; 574kD(Calculated).
蛋白号: Q5T4S7
RRID: AB_2846678

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
UBR4 Antibody detects endogenous levels of total UBR4.
RRID:
AB_2846678
引用格式: Affinity Biosciences Cat# DF13659, RRID:AB_2846678.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

600 kDa retinoblastoma protein associated factor; 600 kDa retinoblastoma protein-associated factor; E3 ubiquitin-protein ligase UBR4; KIAA0462; KIAA1307; N-recognin-4; p600; RBAF600; Retinoblastoma associated factor 600 like protein; Retinoblastoma associated factor of 600 kDa; Retinoblastoma-associated factor 600; Retinoblastoma-associated factor of 600 kDa; Ubiquitin protein ligase E3 component n recognin 4; UBR4; UBR4_HUMAN; Zinc finger UBR1 type protein 1; Zinc finger UBR1-type protein 1; Zinc finger, UBR1 type 1; ZUBR 1; ZUBR1;

抗原和靶标

免疫原:
Uniprot:

Q5T4S7(UBR4_HUMAN) >>Visit HPA database.

基因/基因ID:
UBR4(23352)
序列:
MATSGGEEAAAAAPAPGTPATGADTTPGWEVAVRPLLSASYSAFEMKELPQLVASVIESESEILHHEKQYEPFYSSFVALSTHYITTVCSLIPRNQLQSVAAACKVLIEFSLLRLENPDEACAVSQKHLILLIKGLCTGCSRLDRTEIITFTAMMKSAKLPQTVKTLSDVEDQKELASPVSPELRQKEVQMNFLNQLTSVFNPRTVASQPISTQTLVEGENDEQSSTDQASAIKTKNVFIAQNVASLQELGGSEKLLRVCLNLPYFLRYINRFQDAVLANSFFIMPATVADATAVRNGFHSLVIDVTMALDTLSLPVLEPLNPSRLQDVTVLSLSCLYAGVSVATCMAILHVGSAQQVRTGSTSSKEDDYESDAATIVQKCLEIYDMIGQAISSSRRAGGEHYQNFQLLGAWCLLNSLFLILNLSPTALADKGKEKDPLAALRVRDILSRTKEGVGSPKLGPGKGHQGFGVLSVILANHAIKLLTSLFQDLQVEALHKGWETDGPPAALSIMAQSTSIQRIQRLIDSVPLMNLLLTLLSTSYRKACVLQRQRKGSMSSDASASTDSNTYYEDDFSSTEEDSSQDDDSEPILGQWFEETISPSKEKAAPPPPPPPPPLESSPRVKSPSKQAPGEKGNILASRKDPELFLGLASNILNFITSSMLNSRNNFIRNYLSVSLSEHHMATLASIIKEVDKDGLKGSSDEEFAAALYHFNHSLVTSDLQSPNLQNTLLQQLGVAPFSEGPWPLYIHPQSLSVLSRLLLIWQHKASAQGDPDVPECLKVWDRFLSTMKQNALQGVVPSETEDLNVEHLQMLLLIFHNFTETGRRAILSLFVQIIQELSVNMDAQMRFVPLILARLLLIFDYLLHQYSKAPVYLFEQVQHNLLSPPFGWASGSQDSNSRRATTPLYHGFKEVEENWSKHFSSDAVPHPRFYCVLSPEASEDDLNRLDSVACDVLFSKLVKYDELYAALTALLAAGSQLDTVRRKENKNVTALEACALQYYFLILWRILGILPPSKTYINQLSMNSPEMSECDILHTLRWSSRLRISSYVNWIKDHLIKQGMKAEHASSLLELASTTKCSSVKYDVEIVEEYFARQISSFCSIDCTTILQLHEIPSLQSIYTLDAAISKVQVSLDEHFSKMAAETDPHKSSEITKNLLPATLQLIDTYASFTRAYLLQNFNEEGTTEKPSKEKLQGFAAVLAIGSSRCKANTLGPTLVQNLPSSVQTVCESWNNINTNEFPNIGSWRNAFANDTIPSESYISAVQAAHLGTLCSQSLPLAASLKHTLLSLVRLTGDLIVWSDEMNPPQVIRTLLPLLLESSTESVAEISSNSLERILGPAESDEFLARVYEKLITGCYNILANHADPNSGLDESILEECLQYLEKQLESSQARKAMEEFFSDSGELVQIMMATANENLSAKFCNRVLKFFTKLFQLTEKSPNPSLLHLCGSLAQLACVEPVRLQAWLTRMTTSPPKDSDQLDVIQENRQLLQLLTTYIVRENSQVGEGVCAVLLGTLTPMATEMLANGDGTGFPELMVVMATLASAGQGAGHLQLHNAAVDWLSRCKKYLSQKNVVEKLNANVMHGKHVMILECTCHIMSYLADVTNALSQSNGQGPSHLSVDGEERAIEVDSDWVEELAVEEEDSQAEDSDEDSLCNKLCTFTITQKEFMNQHWYHCHTCKMVDGVGVCTVCAKVCHKDHEISYAKYGSFFCDCGAKEDGSCLALVKRTPSSGMSSTMKESAFQSEPRISESLVRHASTSSPADKAKVTISDGKVADEEKPKKSSLCRTVEGCREELQNQANFSFAPLVLDMLNFLMDAIQTNFQQASAVGSSSRAQQALSELHTVEKAVEMTDQLMVPTLGSQEGAFENVRMNYSGDQGQTIRQLISAHVLRRVAMCVLSSPHGRRQHLAVSHEKGKITVLQLSALLKQADSSKRKLTLTRLASAPVPFTVLSLTGNPCKEDYLAVCGLKDCHVLTFSSSGSVSDHLVLHPQLATGNFIIKAVWLPGSQTELAIVTADFVKIYDLCVDALSPTFYFLLPSSKIRDVTFLFNEEGKNIIVIMSSAGYIYTQLMEEASSAQQGPFYVTNVLEINHEDLKDSNSQVAGGGVSVYYSHVLQMLFFSYCQGKSFAATISRTTLEVLQLFPINIKSSNGGSKTSPALCQWSEVMNHPGLVCCVQQTTGVPLVVMVKPDTFLIQEIKTLPAKAKIQDMVAIRHTACNEQQRTTMILLCEDGSLRIYMANVENTSYWLQPSLQPSSVISIMKPVRKRKTATITTRTSSQVTFPIDFFEHNQQLTDVEFGGNDLLQVYNAQQIKHRLNSTGMYVANTKPGGFTIEISNNNSTMVMTGMRIQIGTQAIERAPSYIEIFGRTMQLNLSRSRWFDFPFTREEALQADKKLNLFIGASVDPAGVTMIDAVKIYGKTKEQFGWPDEPPEEFPSASVSNICPSNLNQSNGTGDSDSAAPTTTSGTVLERLVVSSLEALESCFAVGPIIEKERNKNAAQELATLLLSLPAPASVQQQSKSLLASLHTSRSAYHSHKDQALLSKAVQCLNTSSKEGKDLDPEVFQRLVITARSIAIMRPNNLVHFTESKLPQMETEGMDEGKEPQKQLEGDCCSFITQLVNHFWKLHASKPKNAFLAPACLPGLTHIEATVNALVDIIHGYCTCELDCINTASKIYMQMLLCPDPAVSFSCKQALIRVLRPRNKRRHVTLPSSPRSNTPMGDKDDDDDDDADEKMQSSGIPNGGHIRQESQEQSEVDHGDFEMVSESMVLETAENVNNGNPSPLEALLAGAEGFPPMLDIPPDADDETMVELAIALSLQQDQQGSSSSALGLQSLGLSGQAPSSSSLDAGTLSDTTASAPASDDEGSTAATDGSTLRTSPADHGGSVGSESGGSAVDSVAGEHSVSGRSSAYGDATAEGHPAGPGSVSSSTGAISTTTGHQEGDGSEGEGEGETEGDVHTSNRLHMVRLMLLERLLQTLPQLRNVGGVRAIPYMQVILMLTTDLDGEDEKDKGALDNLLSQLIAELGMDKKDVSKKNERSALNEVHLVVMRLLSVFMSRTKSGSKSSICESSSLISSATAAALLSSGAVDYCLHVLKSLLEYWKSQQNDEEPVATSQLLKPHTTSSPPDMSPFFLRQYVKGHAADVFEAYTQLLTEMVLRLPYQIKKITDTNSRIPPPVFDHSWFYFLSEYLMIQQTPFVRRQVRKLLLFICGSKEKYRQLRDLHTLDSHVRGIKKLLEEQGIFLRASVVTASSGSALQYDTLISLMEHLKACAEIAAQRTINWQKFCIKDDSVLYFLLQVSFLVDEGVSPVLLQLLSCALCGSKVLAALAASSGSSSASSSSAPVAASSGQATTQSKSSTKKSKKEEKEKEKDGETSGSQEDQLCTALVNQLNKFADKETLIQFLRCFLLESNSSSVRWQAHCLTLHIYRNSSKSQQELLLDLMWSIWPELPAYGRKAAQFVDLLGYFSLKTPQTEKKLKEYSQKAVEILRTQNHILTNHPNSNIYNTLSGLVEFDGYYLESDPCLVCNNPEVPFCYIKLSSIKVDTRYTTTQQVVKLIGSHTISKVTVKIGDLKRTKMVRTINLYYNNRTVQAIVELKNKPARWHKAKKVQLTPGQTEVKIDLPLPIVASNLMIEFADFYENYQASTETLQCPRCSASVPANPGVCGNCGENVYQCHKCRSINYDEKDPFLCNACGFCKYARFDFMLYAKPCCAVDPIENEEDRKKAVSNINTLLDKADRVYHQLMGHRPQLENLLCKVNEAAPEKPQDDSGTAGGISSTSASVNRYILQLAQEYCGDCKNSFDELSKIIQKVFASRKELLEYDLQQREAATKSSRTSVQPTFTASQYRALSVLGCGHTSSTKCYGCASAVTEHCITLLRALATNPALRHILVSQGLIRELFDYNLRRGAAAMREEVRQLMCLLTRDNPEATQQMNDLIIGKVSTALKGHWANPDLASSLQYEMLLLTDSISKEDSCWELRLRCALSLFLMAVNIKTPVVVENITLMCLRILQKLIKPPAPTSKKNKDVPVEALTTVKPYCNEIHAQAQLWLKRDPKASYDAWKKCLPIRGIDGNGKAPSKSELRHLYLTEKYVWRWKQFLSRRGKRTSPLDLKLGHNNWLRQVLFTPATQAARQAACTIVEALATIPSRKQQVLDLLTSYLDELSIAGECAAEYLALYQKLITSAHWKVYLAARGVLPYVGNLITKEIARLLALEEATLSTDLQQGYALKSLTGLLSSFVEVESIKRHFKSRLVGTVLNGYLCLRKLVVQRTKLIDETQDMLLEMLEDMTTGTESETKAFMAVCIETAKRYNLDDYRTPVFIFERLCSIIYPEENEVTEFFVTLEKDPQQEDFLQGRMPGNPYSSNEPGIGPLMRDIKNKICQDCDLVALLEDDSGMELLVNNKIISLDLPVAEVYKKVWCTTNEGEPMRIVYRMRGLLGDATEEFIESLDSTTDEEEDEEEVYKMAGVMAQCGGLECMLNRLAGIRDFKQGRHLLTVLLKLFSYCVKVKVNRQQLVKLEMNTLNVMLGTLNLALVAEQESKDSGGAAVAEQVLSIMEIILDESNAEPLSEDKGNLLLTGDKDQLVMLLDQINSTFVRSNPSVLQGLLRIIPYLSFGEVEKMQILVERFKPYCNFDKYDEDHSGDDKVFLDCFCKIAAGIKNNSNGHQLKDLILQKGITQNALDYMKKHIPSAKNLDADIWKKFLSRPALPFILRLLRGLAIQHPGTQVLIGTDSIPNLHKLEQVSSDEGIGTLAENLLEALREHPDVNKKIDAARRETRAEKKRMAMAMRQKALGTLGMTTNEKGQVVTKTALLKQMEELIEEPGLTCCICREGYKFQPTKVLGIYTFTKRVALEEMENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWVESAFEVDGPYYFTVLALHILPPEQWRATRVEILRRLLVTSQARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDVAGLLSEITDPESFLKDLLNSVP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
75
Horse
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q5T4S7 作为底物

Site PTM Type Enzyme
T18 Phosphorylation
S59 Phosphorylation
S61 Phosphorylation
S111 Phosphorylation
K127 Ubiquitination
K159 Ubiquitination
K165 Ubiquitination
K174 Ubiquitination
S178 Phosphorylation
S181 Phosphorylation
K187 Ubiquitination
K234 Ubiquitination
K236 Ubiquitination
K255 Ubiquitination
T360 Phosphorylation
S362 Phosphorylation
T363 Phosphorylation
S364 Phosphorylation
S365 Phosphorylation
K366 Ubiquitination
Y370 Phosphorylation
S372 Phosphorylation
T376 Phosphorylation
Y385 Phosphorylation
K436 Ubiquitination
S457 Phosphorylation
K459 Ubiquitination
T485 Phosphorylation
S486 Phosphorylation
S555 Phosphorylation
S557 Phosphorylation
K605 Ubiquitination
S619 Phosphorylation
S620 Phosphorylation
S625 Phosphorylation
S627 Phosphorylation
K634 Ubiquitination
S665 Phosphorylation
K781 Ubiquitination
T904 Phosphorylation
T905 Phosphorylation
K920 Ubiquitination
Y963 Phosphorylation
Y1001 Phosphorylation
Y1002 Phosphorylation
K1060 Ubiquitination
K1064 Ubiquitination
K1079 Ubiquitination
K1084 Acetylation
K1084 Ubiquitination
K1141 Ubiquitination
K1150 Ubiquitination
K1156 Ubiquitination
K1189 Ubiquitination
K1477 Ubiquitination
K1574 Ubiquitination
K1579 Ubiquitination
S1634 Phosphorylation
S1647 Phosphorylation
S1652 Phosphorylation
K1729 Ubiquitination
T1731 Phosphorylation
S1733 Phosphorylation
T1739 Phosphorylation
K1741 Ubiquitination
S1747 Phosphorylation
S1752 Phosphorylation
S1754 Phosphorylation
S1760 Phosphorylation
T1761 Phosphorylation
S1762 Phosphorylation
S1763 Phosphorylation
K1769 Ubiquitination
S1773 Phosphorylation
K1776 Ubiquitination
S1878 Phosphorylation
T1884 Phosphorylation
S1904 Phosphorylation
K1931 Acetylation
K1931 Ubiquitination
K1939 Ubiquitination
T1941 Phosphorylation
T1943 Phosphorylation
S1947 Phosphorylation
T1958 Phosphorylation
Y2087 Phosphorylation
S2131 Phosphorylation
T2140 Phosphorylation
T2196 Phosphorylation
T2204 Phosphorylation
K2210 Ubiquitination
R2227 Methylation
Y2242 Phosphorylation
K2273 Ubiquitination
K2502 Ubiquitination
Y2539 Phosphorylation
S2541 Phosphorylation
K2543 Ubiquitination
S2549 Phosphorylation
K2550 Ubiquitination
K2560 Ubiquitination
K2563 Ubiquitination
K2698 Ubiquitination
T2715 Phosphorylation
S2718 Phosphorylation
S2719 Phosphorylation
S2722 Phosphorylation
T2724 Phosphorylation
S2743 Phosphorylation
S2756 Phosphorylation
T2884 Phosphorylation
S2885 Phosphorylation
S2892 Phosphorylation
S2895 Phosphorylation
S2897 Phosphorylation
S2900 Phosphorylation
S2910 Phosphorylation
Y2918 Phosphorylation
S2932 Phosphorylation P42345 (MTOR)
S2934 Phosphorylation
T2937 Phosphorylation
T2942 Phosphorylation
T2943 Phosphorylation
T2944 Phosphorylation
S2952 Phosphorylation
T2960 Phosphorylation
K3126 Ubiquitination
S3132 Phosphorylation
Y3169 Phosphorylation
T3175 Phosphorylation
T3177 Phosphorylation
S3179 Phosphorylation
S3220 Phosphorylation
K3292 Ubiquitination
S3339 Phosphorylation
S3346 Phosphorylation
S3347 Phosphorylation
S3349 Phosphorylation
T3361 Phosphorylation
S3366 Phosphorylation
T3367 Phosphorylation
K3379 Ubiquitination
S3386 Phosphorylation
K3401 Ubiquitination
K3405 Ubiquitination
K3464 Ubiquitination
K3485 Ubiquitination
K3487 Ubiquitination
K3551 Ubiquitination
K3573 Ubiquitination
K3577 Ubiquitination
K3617 Ubiquitination
K3745 Ubiquitination
K3774 Ubiquitination
K3808 Ubiquitination
K3816 Ubiquitination
K3820 Ubiquitination
K3826 Ubiquitination
S3842 Phosphorylation
S3854 Phosphorylation
S3860 Phosphorylation
T3892 Phosphorylation
T4005 Phosphorylation
T4013 Phosphorylation
K4035 Ubiquitination
K4065 Ubiquitination
Y4068 Phosphorylation
K4072 Ubiquitination
R4078 Methylation
K4089 Ubiquitination
K4100 Ubiquitination
K4106 Ubiquitination
S4117 Phosphorylation
K4122 Ubiquitination
R4158 Methylation
K4215 Ubiquitination
T4300 Phosphorylation
T4316 Phosphorylation
Y4325 Phosphorylation
S4416 Phosphorylation
K4427 Ubiquitination
R4491 Methylation
R4496 Methylation
K4685 Ubiquitination
K4696 Ubiquitination
K4703 Ubiquitination
K4711 Ubiquitination
K4712 Ubiquitination
S4755 Phosphorylation
T4762 Phosphorylation
K4802 Ubiquitination
K4814 Ubiquitination
T4819 Phosphorylation
K4820 Ubiquitination
K4860 Ubiquitination
S4973 Phosphorylation
S4975 Phosphorylation
T4978 Phosphorylation
K5022 Ubiquitination
Y5135 Phosphorylation

研究背景

功能:

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Mediates ubiquitination of ACLY, leading to its subsequent degradation.

细胞定位:

Membrane>Multi-pass membrane protein. Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus.
Note: Concentrates at the leading edge of membrane structures involved in actin motility.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with RB1 and calmodulin.

(Microbial infection) Interacts with protein E7 from papilloma virus HPV-16, HPV-6B and HPV-11.

蛋白家族:

Belongs to the UBR4 family.

研究领域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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