JAK2 Antibody - #AF6022

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产品: JAK2 抗体
货号: AF6022
描述: Rabbit polyclonal antibody to JAK2
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: O60674
RRID: AB_2834956

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
JAK2 Antibody detects endogenous levels of total JAK2.
RRID:
AB_2834956
引用格式: Affinity Biosciences Cat# AF6022, RRID:AB_2834956.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

JAK 2; JAK-2; JAK2; JAK2_HUMAN; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; JTK 10; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2;

抗原和靶标

免疫原:

A synthesized peptide derived from human JAK2, corresponding to a region within C-terminal amino acids.

基因/基因ID:
JAK2(3717)
描述:
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Lacc1-engineered extracellular vesicles reprogram mitochondrial metabolism to alleviate inflammation and cartilage degeneration in TMJ osteoarthritis. Journal of nanobiotechnology, 2025 (PubMed: 40186254) [IF=10.2]
2). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB    Species: Mouse    Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p 
3). Lactate Facilitates Pancreatic Repair Following Acute Pancreatitis by Promoting Reparative Macrophage Polarization. Cellular and Molecular Gastroenterology and Hepatology, 2025 [IF=7.4]

Application: WB    Species: Mouse    Sample:

Figure 12. Lactate inhibits the JAK2-STAT1 signaling pathway in macrophages through the GPR132 receptor, facilitating a switch in macrophage phenotype. (A) KEGG pathway analysis was conducted on significantly differentially expressed genes in pancreatic macrophages during recovery following AP in WT mice, alongside the mRNA expression levels of Gpr132, n = 3/group. (B) qPCR analysis of Gpr132 mRNA expression in macrophages under lactate and GPR132 antagonist (0.075 μM, 0.15 μM, or 0.3 μM) treatment, Lactate (Lac) = 15 mM, n = 3–4/group. (C–D) WB analysis was performed to assess the expression levels of β-actin, STAT1, P-STAT1, JAK2, P-JAK2 (C), with the relative quantification (D), n = 3/group, Gpr = 0.075 μM. (E) qPCR detection of pro-inflammatory genes (Cxcl10, Ccl12, Il6) expression levels in macrophages following intervention with a GPR132 inhibitor, n = 3–4/group. (F) qPCR analysis of repair genes (Lrg1, Retnla, Vegfα) expression levels in macrophages post-intervention with a GPR132 inhibitor, n = 3–4/group. Data are expressed as mean ± SEM. Statistical significance is indicated as follows: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; and ∗∗∗∗P < .0001.
4). MiR-15b is a key regulator of proliferation and apoptosis of chondrocytes from patients with condylar hyperplasia by targeting IGF1, IGF1R and BCL2. Osteoarthritis and Cartilage, 2019 (PubMed: 30521861) [IF=7.2]
5). Phillygenin inhibited M1 macrophage polarization and reduced hepatic stellate cell activation by inhibiting macrophage exosomal miR-125b-5p. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36652738) [IF=6.9]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Fig. 2. PHI inhibited the JAK/STAT and Notch1 signaling pathways in RAW264.7 cells. (a) The 3D and 2D models of molecular docking of PHI and JAK1. (b) The 3D and 2D models of molecular docking of PHI and JAK2. (c) The 3D and 2D models of molecular docking of PHI and Notch1. (d-g) The expression of JAK1, JAK2, STAT1 and Notch1 mRNA in RAW264.7 cells after treatment with LPS/IFNγ and PHI for 12 h was detected by RT-qPCR (n = 3). (h) The expression of JAK1, JAK2, p-JAK1, p-JAK2, STAT1, p-STAT1 and Notch1 proteins in RAW264.7 cells after treatment with LPS/IFNγ and PHI for 12 h was detected by western blotting. (i-l) The relative quantification of p-JAK1/JAK1, p-JAK2/JAK2, p-STAT1/STAT1, and Notch1 protein expression in western blotting results was analyzed by ImageJ software (n = 3). Results are presented as mean ± SD. ###P 
6). Formononetin protects against inflammation associated with cerebral ischemia-reperfusion injury in rats by targeting the JAK2/STAT3 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35339827) [IF=6.9]
7). Targeted pharmacokinetics and bioinformatics screening strategy reveals JAK2 as the main target for Xin-Ji-Er-Kang in treatment of MIR injury. Biomedicine & Pharmacotherapy, 2022 (PubMed: 36271569) [IF=6.9]

Application: WB    Species: Human    Sample:

Fig. 5. Effects of XJEK on JAK2/STAT1/STAT3 pathway and myocardial cell apoptosis after MIR injury. A Representative figure of JAK2, pJAK2, STAT3, pSTAT3, STAT1, pSTAT1 protein. B-E Quantitative analyses of JAK2, pJAK2, STAT3, pSTAT3, STAT1, pSTAT1 protein (n = 6). F Representative photographs of TUNEL/DAPI double staining of the cardiomyocytes after MIR (×630). H The quantitative analysis of TUNEL/DAPI (n = 6). G Representative figures of expression of Bax and Bcl-2. I Quantitative analyses of Bax and Bcl-2 protein (n = 6). *P 
8). β-elemene alleviates hyperglycemia-induced cardiac inflammation and remodeling by inhibiting the JAK/STAT3-NF-κB pathway. Phytomedicine, 2023 (PubMed: 37531901) [IF=6.7]
9). Higenamine alleviates allergic rhinitis by activating AKT1 and suppressing the EGFR/JAK2/c-JUN signaling. PHYTOMEDICINE, 2021 (PubMed: 33945919) [IF=6.7]

Application: WB    Species: Human    Sample: HNEpCs

Fig. 8. The effects of higenamine (HG) on potential targets in histamine-induced HNEpCs. (A-J) Levels of protein expression of AKT1, p-AKT1, EGFR, p-EGFR, c-Jun, p-c-Jun, iNOS, JAK2, and p-JAK2 were determined by Western blotting, β-actin was used as an internal control. Data are expressed as means ± SD of three experiments. ## p < 0.01, and # p < 0.05 compared with the control group. ** p < 0.01, and * p < 0.05 compared with the histamine group.
10). Design, synthesis and biological evaluation of N-anthraniloyl tryptamine derivatives as pleiotropic molecules for the therapy of malignant glioma. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34091208) [IF=6.0]

Application: WB    Species: Human    Sample: U251 and U87MG cells

Fig. 2. NP16 down-regulated the expression of COX-2, p-JAK2 and p-STAT3, and inhibited the nuclear translocation of STAT3 in both U251 and U87MG cells. (A, B) western blot analysis of the expression of JAK2, p-JAK2, p-STAT3 and STAT3: the cells were exposed to NP16 at concentrations of 0.25, 1 and 4 mM for 8 h; western blot analysis of the expression of COX-2: the cells were exposed to NP16 at concentrations of 1, 2 and 4 mM for 24 h (C, D, E) Relative expression of the target protein in the cell lysate was calculated using the ImageJ image analysis software. Each error bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus the DMSO group. (F, G) representative fluorescence microscopy images: the cells were exposed to NP16 at concentrations of 4 mM for 8 h. Compound NP16 inhibited nuclear translocation of STAT3 in both U251 and U87MG cells. (Magnification  60, scale bar ¼ 20 mm).
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