Survivin Antibody - #AF6017

Fig. 5 The IL6-STAT3 pathway promoted prostate cancer docetaxel chemoresistance. A Western blot of relative proteins in RM-1 and DU-145 cultured with different conditions. B, C Cell viability and TUNEL assay (scale bar, 100 μm) were conducted on RM-1 and DU-145 under condition as described. D Flowchart of the NC, Docetaxel, Abx+Docetaxel, and Abx+Docetaxel+Stattic groups for in vivo study. Relevant tumor images and comparison of volume and weight for tumors in four groups (n = 5). E Immunohistochemistry of tumor tissues for p-STAT3-, Bcl-2-, and survivin-positive cell in four groups (scale bar, 50 μm). Statistical significance was assessed by LSD in one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001

Fig. 5 The IL6-STAT3 pathway promoted prostate cancer docetaxel chemoresistance. A Western blot of relative proteins in RM-1 and DU-145 cultured with different conditions. B, C Cell viability and TUNEL assay (scale bar, 100 μm) were conducted on RM-1 and DU-145 under condition as described. D Flowchart of the NC, Docetaxel, Abx+Docetaxel, and Abx+Docetaxel+Stattic groups for in vivo study. Relevant tumor images and comparison of volume and weight for tumors in four groups (n = 5). E Immunohistochemistry of tumor tissues for p-STAT3-, Bcl-2-, and survivin-positive cell in four groups (scale bar, 50 μm). Statistical significance was assessed by LSD in one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001

Fig. 6. |DHEA suppressed STAT3/NFAT signal pathway in lung. A, PY750-STAT3 and STAT3; B, NFATc2; C, Pim-1; D, Survivin. The top shows representative immunoblots, and the bottom shows the densitometric assessment. The values are means ± SE, n = 5 rats per group; *P < 0.05 versus the sham group, #P < 0.05 versus the PH-LHF group.

Figure 3 Cell invasion assays and expression analysis of Wnt/EMT related genes in cells treated with gigantol. (A) Cell invasion was measured by transwell assay in bladder cancer cells treated with increasing concentrations of gigantol (magnification ×100). (B) qRT-PCR analysis of the Wnt target genes and EMT markers in bladder cancer cells treated with gigantol. (C) Western blot analysis of Wnt/EMT markers in bladder cancer cells treated with indicated concentrations of gigantol. Data in (B) were shown as means ± SD (n = 3), the statistically significant differences were considered at *p<0.05, **p<0.01, ***p<0.001.

FIG. 6. The Wnt/b-catenin signaling pathway protein expression level in A549 and H460 cell lines detected by Western blot. *p < 0.05, compared with si-NC group, # p < 0.05, in contrast to si-NEAT1+NC inhibitor group.