SLFN14 Antibody - #DF13579

Figure 3 T853fs leads to low expression level of SLFN14 (A and B) HEK293T and Meg-01 cells were transfected with SLFN14-WT or SLFN14-T853fs overexpression plasmids for 48 h. The expressions of exogenous SLFN14 were determined by (A) real time qPCR and (B) western blot. Both representative figures and quantification data for western blot are shown. GAPDH was used as an internal reference. (C) Western blot analysis of SLFN14 protein expression in platelet lysates of the proband and two healthy subjects (control 1 and control 2). Both representative figures and quantification data are shown. (D) rRNA degradation in SLFN14-WT and SLFN14-T853fs) overexpressing HEK293T cells was assessed by denaturing agarose/formaldehyde gel electrophoresis (n = 3 independent experiments). (E) Cell proliferation of MEG-01 cells that were transfected by empty vector (EV), SLFN14-WT, or SLFN14-T853fs overexpression plasmids was determined by Cell Counting Kit-8 (CCK-8) assay. (F) Immunofluorescence staining against SLFN14 (red) of MEG-01 cells transfected with SLFN14-WT or SLFN14-T853fs overexpression plasmids for 48 h. Nuclei were counterstained with DAPI (blue). Merged images indicate the overlay of SLFN14 and nuclei signals. Images were captured using a 60× objective. Scale bars: 20 μm. Data are presented as mean ± standard deviation (SD). Comparisons between two groups were conducted using unpaired two-tailed Student’s t test.