DBT Antibody - #DF13569

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产品: DBT 抗体
货号: DF13569
描述: Rabbit polyclonal antibody to DBT
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 53kDa; 53kD(Calculated).
蛋白号: P11182
RRID: AB_2846588

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
DBT Antibody detects endogenous levels of total DBT.
RRID:
AB_2846588
引用格式: Affinity Biosciences Cat# DF13569, RRID:AB_2846588.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BCATE2; BCKAD E2 subunit; Branched chain acyltransferase, E2 component; Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease); Dihydrolipoamide branched chain transacylase; Dihydrolipoamide branched chain transacylase E2; Dihydrolipoyl transacylase; Dihydrolipoyllysine residue (2 methylpropanoyl)transferase; E2; E2 component of branched chain alpha keto acid dehydrogenase complex; E2B; Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex; Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b);

抗原和靶标

免疫原:
Uniprot:

P11182(ODB2_HUMAN) >>Visit HPA database.

基因/基因ID:
DBT(1629)
序列:
MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
86
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11182 作为底物

Site PTM Type Enzyme
T56 Phosphorylation
T57 Phosphorylation
T80 Phosphorylation
T153 Phosphorylation
T171 Phosphorylation
K202 Ubiquitination
T213 Phosphorylation
S220 Phosphorylation
K289 Methylation
K295 Acetylation
T392 Phosphorylation
S403 Phosphorylation
K435 Acetylation
K435 Ubiquitination
K440 Acetylation
S447 Phosphorylation
S469 Phosphorylation

研究背景

功能:

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms a 24-polypeptide structural core with octahedral symmetry.

蛋白家族:

Belongs to the 2-oxoacid dehydrogenase family.

研究领域

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). A comprehensive analysis of the prognostic value and immune infiltration of low expression DBT in clear cell renal cell carcinoma. Frontiers in Pharmacology (PubMed: 36299888) [IF=5.6]

Application: WB    Species: Human    Sample: HK-2 cells

FIGURE 2 DBT protein expression is low in ccRCC. (A) The histogram obtained from the analysis of data from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) on the UALCAN website shows significantly lower expression of the DBT protein in ccRCC tissues versus normal tissues (p = 1.31015316430268E-57). (B) The results of western blotting of A498, 786-O, ACHN, Caki-1, and OSRC-2 cell lysates showed lower protein expression of DBT in ccRCC cells compared to HK-2 cells, and the histogram quantifying the results also indicated a statistically significant difference. (C) Similarly, DBT protein expression was lower in renal clear cell carcinoma tissues than in matched pairs of paraneoplastic normal tissues, and the histogram quantification confirmed this finding. (D) Immunohistochemical results from the HPA database showed that DBT expression in ccRCC was mainly in the nucleus, whereas in normal renal tissue, it was in normal tubular epithelial cells. (E) This result was also validated by the immunohistochemical results of 2 pairs of ccRCC tissues from our study centre.

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