DNA Polymerase theta Antibody - #DF13563

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产品: DNA Polymerase theta 抗体
货号: DF13563
描述: Rabbit polyclonal antibody to DNA Polymerase theta
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: O75417
RRID: AB_2846582

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
DNA Polymerase theta Antibody detects endogenous levels of total DNA Polymerase theta.
RRID:
AB_2846582
引用格式: Affinity Biosciences Cat# DF13563, RRID:AB_2846582.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp781A0112; DNA polymerase eta; DNA polymerase theta; DPOLQ_HUMAN; POLQ; Polymerase (DNA directed) theta; PRO0327;

抗原和靶标

免疫原:

A synthesized peptide derived from human DNA Polymerase theta, corresponding to a region within the internal amino acids.

基因/基因ID:
POLQ(10721)

文献引用

1). ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acid receptor-mediated DNA polymerase θ expression. npj Precision Oncology, 2023 (PubMed: 37429899) [IF=7.9]

Application: WB    Species: Human    Sample: PEO1 and OVCAR3 cells

Fig. 1 ALDH1A1 enhances Polθ expression. a Volcano plot depicting differentially expressed MMEJ-related genes in ALDH+ vs ALDH- cells isolated from SKOV3 cells. b Volcano plot depicting differentially expressed MMEJ-related genes in siALDH1A1 transfected vs siControl transfected PEO1 cells. c The POLQ mRNA level is higher in ALDH-br cells than that in ALDH-dim cells isolated from PEO1 and OVCAR3 cells. d Overexpression of ALDH1A1 increased the mRNA level of POLQ. e Overexpression of ALDH1A1 increased the protein level of Polθ. f Knockdown of ALDH1A1 decreased the mRNA level of POLQ. g Knockdown of ALDH1A1 decreased the protein level of Polθ. h Inhibition of the activity of ALDH1A1 with NCT-505 reduced the expression of Polθ. Measurements were taken from distinct samples. N = 3, bar: s.d., **P 

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