Ornithine Carbamoyltransferase Antibody - #DF13552

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产品: Ornithine Carbamoyltransferase 抗体
货号: DF13552
描述: Rabbit polyclonal antibody to Ornithine Carbamoyltransferase
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 39kDa, 55 kDa; 40kD(Calculated).
蛋白号: P00480
RRID: AB_2846571

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Ornithine Carbamoyltransferase Antibody detects endogenous levels of total Ornithine Carbamoyltransferase.
RRID:
AB_2846571
引用格式: Affinity Biosciences Cat# DF13552, RRID:AB_2846571.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.1.3.3; MGC129967; MGC129968; MGC138856; OCTD; Ornithine Carbamoyltransferase; Ornithine carbamoyltransferase mitochondrial; Ornithine carbamoyltransferase, mitochondrial; Ornithine transcarbamylase; Otc; OTC_HUMAN; OTCase;

抗原和靶标

免疫原:
Uniprot:

P00480(OTC_HUMAN) >>Visit HPA database.

基因/基因ID:
OTC(5009)
表达:
P00480 OTC_HUMAN:

Mainly expressed in liver and intestinal mucosa.

序列:
MLFNLRILLNNAAFRNGHNFMVRNFRCGQPLQNKVQLKGRDLLTLKNFTGEEIKYMLWLSADLKFRIKQKGEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPCFLTTQDIHLGVNESLTDTARVLSSMADAVLARVYKQSDLDTLAKEASIPIINGLSDLYHPIQILADYLTLQEHYSSLKGLTLSWIGDGNNILHSIMMSAAKFGMHLQAATPKGYEPDASVTKLAEQYAKENGTKLLLTNDPLEAAHGGNVLITDTWISMGQEEEKKKRLQAFQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPQLQKPKF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P00480 作为底物

Site PTM Type Enzyme
K46 Acetylation
Y55 Phosphorylation
K88 Acetylation
S133 Phosphorylation
S146 Phosphorylation
Y223 Phosphorylation
K231 Acetylation
Y284 Phosphorylation
Y317 Phosphorylation

研究背景

翻译修饰:

Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in liver and intestinal mucosa.

亚基结构:

Homotrimer.

蛋白家族:

Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.

研究领域

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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