SHPS1 Antibody - #AF0253

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产品: SHPS1 抗体
货号: AF0253
描述: Rabbit polyclonal antibody to SHPS1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
蛋白号: P78324
RRID: AB_2833428

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
SHPS1 Antibody detects endogenous levels of total SHPS1.
RRID:
AB_2833428
引用格式: Affinity Biosciences Cat# AF0253, RRID:AB_2833428.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Signal regulatory protein alpha type 1; Bit; Brain Ig like molecule with tyrosine based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; Brain immunoglobulin like molecule with tyrosine based activation motifs; CD172 antigen like family member A; CD172 antigen-like family member A; CD172a; CD172a antigen; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MYD 1; Myd 1 antigen; MyD-1 antigen; p84; Protein tyrosine phosphatase non receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS-1; SHPS1; SHPS1_HUMAN; Signal regulatory protein alpha 2; Signal regulatory protein alpha 3; Signal regulatory protein alpha; Signal regulatory protein alpha type 2; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SIRPA; SIRPalpha; SIRPalpha1; SIRPalpha2; SIRPalpha3; Tyrosine phosphatase SHP substrate 1; Tyrosine protein phosphatase non receptor type substrate 1; Tyrosine-protein phosphatase non-receptor type substrate 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human SHPS1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
SIRPA(140885)
描述:
SHPS1 Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

研究领域

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation. Cell death discovery, 2025 (PubMed: 40050290) [IF=7.0]
2). Characterization of myeloid signature genes for predicting prognosis and immune landscape in Ewing sarcoma. Cancer science, 2023 (PubMed: 36478349) [IF=4.5]

Application: WB    Species: human    Sample: EWS cell

FIGURE 9 In vitro validation of CTSD, SIRPA, and FN1 expression. A, Western blotting showing protein expression levels of CTSD, SIRPA, and FN1 in Ewing sarcoma (EWS) cell lines and normal controls; B-D, Quantitative real-time PCR showing the relative mRNA expression levels of CTSD, SIRPA, and FN1 in EWS cell lines and normal controls. E-G, Representative immunohistochemistry staining of CTSD, SIRPA, and FN1 in EWS tissue and matched para-cancer. H-J, Quantitative analysis of the positive rate of pathological stained sections for these three genes. *p 

Application: IHC    Species: human    Sample: EWS cell

FIGURE 9 In vitro validation of CTSD, SIRPA, and FN1 expression. A, Western blotting showing protein expression levels of CTSD, SIRPA, and FN1 in Ewing sarcoma (EWS) cell lines and normal controls; B-D, Quantitative real-time PCR showing the relative mRNA expression levels of CTSD, SIRPA, and FN1 in EWS cell lines and normal controls. E-G, Representative immunohistochemistry staining of CTSD, SIRPA, and FN1 in EWS tissue and matched para-cancer. H-J, Quantitative analysis of the positive rate of pathological stained sections for these three genes. *p 
3). Exercise modulates polarization of TAMs and expression of related immune checkpoints in mice with lung cancer. Journal of Cancer, 2022 (PubMed: 36186906) [IF=3.3]

Application: WB    Species: Mouse    Sample: lung cancer tissues

Figure 3. The effect of exercise on the expression of immune checkpoints in lung cancer tissues. A: Tumor cell-associated ligand mRNA expression levels (n=8). B: mRNA expression level of the receptor (n=8). C, D, E, F, G: Expression levels of CD47 (C, D), CD24 (C, E), H2KD (C, F) and PD-L1 (C, G) in lung cancer tissues (n=6). C, H, I: Expression levels of SIRPα (C, H) and PD-1 (C, I) in lung cancer tissues (n=6). Note: & represents significant difference compared with Saline group (P

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