| 产品: | BIN1 抗体 |
| 货号: | DF13520 |
| 描述: | Rabbit polyclonal antibody to BIN1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Horse, Rabbit, Dog |
| 分子量: | 64kDa; 65kD(Calculated). |
| 蛋白号: | O00499 |
| RRID: | AB_2846539 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13520, RRID:AB_2846539.
展开/折叠
AMPH 2; AMPH2; Amphiphysin 2; Amphiphysin II; Amphiphysin like protein; amphiphysin-like; Amphiphysin-like protein; AMPHL; Bin1; BIN1_HUMAN; Box Dependant MYC Interacting Protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; Myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3P9;
抗原和靶标
Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
- O00499 BIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQWCQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQFEAPGPFSEQASLLDLDFDPLPPVTSPVKAPTPSGQSIPWDLWEPTESPAGSLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00499 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| R66 | Methylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot |
研究背景
Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.
Phosphorylated by protein kinase C.
Nucleus. Cytoplasm. Endosome. Cell membrane>Sarcolemma>T-tubule.
Cytoplasm.
Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Heterodimer with AMPH (By similarity). Binds SH3GLB1 (By similarity). Interacts (via SH3 domain) with DNM1. Interacts with SYNJ1 (By similarity). Interacts (via SH3 domain) with DNM2. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1 (By similarity). Interacts with MYC (via N-terminal transactivation domain); the interaction requires the integrity of the conserved MYC box regions 1 and 2 (By similarity). Interacts with BIN2. Interacts with SNX4. Interacts (via BAR domain) with BACE1. Binds (via BAR domain) F-actin.
(Microbial infection) Interacts (SH3 domain) with HCV NS5A.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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