Chk1 Antibody - #AF6007

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产品: Chk1 抗体
货号: AF6007
描述: Rabbit polyclonal antibody to Chk1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: O14757
RRID: AB_2834941

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Chk1 Antibody detects endogenous levels of total Chk1.
RRID:
AB_2834941
引用格式: Affinity Biosciences Cat# AF6007, RRID:AB_2834941.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C85740; Cell cycle checkpoint kinase; Checkpoint , S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; Chek1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); CHK1_HUMAN; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae);

抗原和靶标

免疫原:

A synthesized peptide derived from human Chk1, corresponding to a region within the internal amino acids.

基因/基因ID:
CHEK1(1111)
描述:
DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Isoliquiritigenin suppresses the progression of malignant melanoma via targeting H2A.Z.1-E2F1 pathway. Biochemical pharmacology, 2023 (PubMed: 37863326) [IF=5.3]

Application: WB    Species: Human    Sample: A375 and SK-MEL-28 cell

Fig. 2. ISL inhibited cell cycle progression. Two melanoma cell lines (A375 and SK-MEL-28) were treated with different concentrations of ISL (0, 10, 20 μM) for 24 h. (A) Cell cycle distribution analysis by flow cytometry for 48 h. (B) The expression of Cyclin D1, CDK2, CDK4 and CHEK1 in A375 and SK-MEL-28 cell lines with ISL administration for 48 h. The data represented the mean ± SEM of three independent experiments,
2). CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. Scientific Reports, 2023 (PubMed: 36720923) [IF=3.8]

Application: WB    Species: Human    Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Figure 3 CENPF regulates ADR-induced G2/M phase arrest through Chk1. (A) Flow cytometry was performed to determine the effects of CENPF inhibition on cell cycle distribution with or without adriamycin in MDA-MB-231 cells. (B,C) The effect of CENPF inhibition on cell proliferation in MDA-MB-231 (B) or MDA-MB-231/ADR (C) cells. (D) Cell proliferation was determined by EdU assay. (E,F) Using RT‒qPCR, the mRNA level of CHK1 was detected in MDA-MB-231 (E) or MDA-MB-231/ADR (F) cells with CENPF knockdown. (G) Western blot analysis of the effect of CENPF knockdown on Chk1 expression and phosphorylation in MDA-MB-231 and MDA-MB-231/ADR cells exposed to adriamycin. Data shown represent the means (± SD) of three independent experiments; **P 
3). Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells. EXPERIMENTAL CELL RESEARCH, 2018 (PubMed: 29964052) [IF=3.3]
4). CENPF Knockdown Inhibits Adriamycin Chemoresistance in Triple Negative Breast Cancer mediated by Rb-E2F1-Chk1 Axis. Research Square, 2022

Application: WB    Species: Human    Sample:

Figure 3. CENPF regulates ADR-induced G2/M phase arrest through Chk1. A Flow cytometry was performed to determine the effects of CENPF inhibition on cell cycle distribution with or without adriamycin in MDA-MB-231. B-C The effect of CENPF inhibition on cell proliferation activities in MDA-MB-231 (B) or MDA-MB-231/ADR (C). D Cell proliferation was determined by EdU assay. E-F Using RT-qPCR, the mRNA level of CHK1 were detected in MDA-MB-231 (E) or MDA-MB-231/ADR (F) with CENPF knockdown. G Western blot detected the effect of CENPF knockdown on Chk1 expression and its phosphorylation in MDA-MB-231 and MDA-MB-231/ADR exposed to adriamycin. Data shown represent the means (± SD) of three independent experiments; **P < 0.01, ****P < 0.0001; NS, not significant; one-way ANOVA (A-F).

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