MTR Antibody - #DF13489

(2)  
产品: MTR 抗体
货号: DF13489
描述: Rabbit polyclonal antibody to MTR
应用: WB IHC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 140kDa; 141kD(Calculated).
蛋白号: Q99707
RRID: AB_2846508

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
克隆:
Polyclonal
特异性:
MTR Antibody detects endogenous levels of total MTR.
RRID:
AB_2846508
引用格式: Affinity Biosciences Cat# DF13489, RRID:AB_2846508.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5-methyltetrahydrofolate homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase; cblG; Methionine synthase; methioninesynthase; MS; MTR; MTR1; PRP20; SRM1; Vitamin-B12 dependent methionine synthase;

抗原和靶标

免疫原:
Uniprot:

Q99707(METH_HUMAN) >>Visit HPA database.

基因/基因ID:
MTR(4548)
表达:
Q99707 METH_HUMAN:

Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.

序列:
MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDILSITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQNLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTAYVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATAFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Zebrafish
88
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99707 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
K16 Ubiquitination
T17 Phosphorylation
K28 Ubiquitination
T39 Phosphorylation
K45 Ubiquitination
K57 Ubiquitination
K64 Ubiquitination
S121 Phosphorylation
K127 Ubiquitination
T133 Phosphorylation
K139 Ubiquitination
K151 Ubiquitination
K231 Ubiquitination
K309 Ubiquitination
K339 Ubiquitination
K388 Ubiquitination
Y395 Phosphorylation
K466 Ubiquitination
K475 Ubiquitination
K484 Ubiquitination
Y491 Phosphorylation
Y520 Phosphorylation
K565 Ubiquitination
K638 Ubiquitination
K645 Ubiquitination
K658 Ubiquitination
K659 Ubiquitination
Y677 Phosphorylation
K681 Ubiquitination
K685 Ubiquitination
K699 Ubiquitination
K700 Ubiquitination
Y701 Phosphorylation
K727 Ubiquitination
K735 Ubiquitination
K742 Ubiquitination
K753 Ubiquitination
T758 Phosphorylation
T764 Phosphorylation
T774 Phosphorylation
K781 Ubiquitination
K789 Ubiquitination
K814 Ubiquitination
K817 Methylation
K817 Ubiquitination
K823 Ubiquitination
K865 Ubiquitination
K871 Ubiquitination
S877 Phosphorylation
Y905 Phosphorylation
Y913 Phosphorylation
Y921 Phosphorylation
K925 Ubiquitination
Y929 Phosphorylation
K952 Ubiquitination
K995 Ubiquitination
K1000 Ubiquitination
K1008 Ubiquitination
Y1010 Phosphorylation
S1022 Phosphorylation
K1024 Ubiquitination
K1071 Ubiquitination
K1127 Ubiquitination
R1132 Methylation
S1155 Phosphorylation
K1169 Ubiquitination
K1186 Ubiquitination
S1206 Phosphorylation
Y1219 Phosphorylation
S1221 Phosphorylation
K1226 Ubiquitination
K1232 Ubiquitination
K1235 Ubiquitination
K1245 Ubiquitination

研究背景

功能:

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.

亚基结构:

Monomer. Dimer. Mainly monomer. Interacts with MTRR.

蛋白家族:

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin (By similarity).

Belongs to the vitamin-B12 dependent methionine synthase family.

研究领域

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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