产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13467, RRID:AB_2846486.
展开/折叠
D12S1889; KIF 5A; Kif5a; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; MY050; Neuronal kinesin heavy chain; NKHC 1; NKHC; SPG 10;
抗原和靶标
- Q12840 KIF5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12840 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| T807 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| K845 | Ubiquitination | Uniprot | |
| K883 | Acetylation | Uniprot | |
| K888 | Acetylation | Uniprot | |
| K890 | Acetylation | Uniprot | |
| R899 | Methylation | Uniprot | |
| S931 | Phosphorylation | Uniprot |
研究背景
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Perikaryon.
Note: Concentrated in the cell body of the neurons, particularly in the perinuclear region.
Distributed throughout the CNS but is highly enriched in subsets of neurons.
Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner. Interacts with ZFYVE27. Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (By similarity). Interacts with BORCS5. Interacts with BICD2.
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
限制条款
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