HSP20 Antibody - #AF6003

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产品: HSP20 抗体
货号: AF6003
描述: Rabbit polyclonal antibody to HSP20
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 18kDa; 17kD(Calculated).
蛋白号: O14558
RRID: AB_2834938

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(85%), Dog(100%)
克隆:
Polyclonal
特异性:
HSP20 Antibody detects endogenous levels of total HSP20.
RRID:
AB_2834938
引用格式: Affinity Biosciences Cat# AF6003, RRID:AB_2834938.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

epididymis luminal protein 55; FLJ32389; Heat shock 20 kDa like protein p20; Heat shock 20 kDa-like protein p20; Heat shock protein alpha crystallin related B6; Heat shock protein beta 6; Heat shock protein beta-6; Heat shock protein, 20-KD; Heat-shock 27-KD protein 6; HEL55; Hsp20; HspB6; HSPB6_HUMAN;

抗原和靶标

免疫原:
Uniprot:

O14558(HSPB6_HUMAN) >>Visit HPA database.

基因/基因ID:
HSPB6(126393)
描述:
HSP20 a small heat shock protein of the HSP20 family. Note: This description may include information from UniProtKB.
序列:
MEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
85
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O14558 作为底物

Site PTM Type Enzyme
S16 Phosphorylation Q15139 (PRKD1) , P17612 (PRKACA)
S59 Phosphorylation
S84 Phosphorylation

研究背景

功能:

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.

翻译修饰:

The N-terminus is blocked.

Phosphorylated at Ser-16 by PKA and probably PKD1K; required to protect cardiomyocytes from apoptosis.

细胞定位:

Cytoplasm. Nucleus. Secreted.
Note: Translocates to nuclear foci during heat shock.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Small heat shock proteins form high molecular mass oligomers containing variable number of monomers; these oligomers display a very flexible quaternary structure easily exchanging their subunits. Heterooligomer with HSPB1; formed through oligomerization of HSPB1:HSBP6 dimers; subunit exchange leads to formation of at least two different heterooligomeric complexes, differing in variable quantities of HSPB1 and HSPB6 homodimers in addition to HSPB1:HSPB6 heterodimers. Heterooligomer with CRYAB; large heterooligomers consist of CRYAB homodimers and HSPB5:HSPB6 heterodimers but lacking HSPB6 homodimers. Interacts with BAG3. Interacts (phosphorylated) with YWHAZ. Interacts with PDE4A and PDE4D; required for maintenance of the non-phosphorylated state of HSPB6 under basal conditions. Interacts with KDR. Interacts with PRKD1.

蛋白家族:

Belongs to the small heat shock protein (HSP20) family.

文献引用

1). Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. Oncology Letters (PubMed: 36380873) [IF=2.9]

Application: WB    Species: Human    Sample: MDA-MB-468 cell

Figure 2. HSP20 overexpression inhibits BC cell proliferation. (A) Relative mRNA and protein expression levels of HSP20 in five BC cell lines compared with the MCF-10A normal mammary epithelial cell line. (B) Relative expression levels of HSP20 in MDA-MB-231 and MDA-MB-468 cells overexpressing HSP20. (C) Cell Counting Kit-8 proliferation assay. (D) Representative images and quantitative analysis of colony formation assays. Data are presented as the mean ± SD. **P<0.01; ***P<0.001 vs. MCF-10A or as indicated. BC, breast cancer; control, untransfected cells; vector, cells transfected with an empty vector; exHSP20, HSP20-overexpressing cells; HSP20, heat shock protein 20; OD, optical density.

Application: IHC    Species: Human    Sample: MDA-MB-468 cell

Figure 2. HSP20 overexpression inhibits BC cell proliferation. (A) Relative mRNA and protein expression levels of HSP20 in five BC cell lines compared with the MCF-10A normal mammary epithelial cell line. (B) Relative expression levels of HSP20 in MDA-MB-231 and MDA-MB-468 cells overexpressing HSP20. (C) Cell Counting Kit-8 proliferation assay. (D) Representative images and quantitative analysis of colony formation assays. Data are presented as the mean ± SD. **P<0.01; ***P<0.001 vs. MCF-10A or as indicated. BC, breast cancer; control, untransfected cells; vector, cells transfected with an empty vector; exHSP20, HSP20-overexpressing cells; HSP20, heat shock protein 20; OD, optical density.

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