JAK1 Antibody - #AF5012

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产品: JAK1 抗体
货号: AF5012
描述: Rabbit polyclonal antibody to JAK1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P23458
RRID: AB_2834933

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
JAK1 Antibody detects endogenous levels of total JAK1.
RRID:
AB_2834933
引用格式: Affinity Biosciences Cat# AF5012, RRID:AB_2834933.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1;

抗原和靶标

免疫原:

A synthesized peptide derived from human JAK1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
JAK1(3716)
描述:
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

文献引用

1). A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39679768) [IF=13.0]
2). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB    Species: Mice    Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)
3). Effects of Cyhalofop-butyl on the Developmental Toxicity and Immunotoxicity in Zebrafish (Danio rerio). Chemosphere, 2021 (PubMed: 33297003) [IF=8.1]

Application: WB    Species: Fish    Sample: zebrafish larvae

Fig. 5. Cyhalofop-butyl up-regulated the expression of the JAK-STAT signaling pathway related genes in zebrafish larvae. (A) At 96 hpf, the mRNA expression patterns of JAK1, STAT3, SOCS3b, TNF-a, CXCL-C1C, IL-6 and IL-1b in zebrafish larvae exposed to 0.5 mg/L cyhalofop-butyl (n ¼ 3, mean ± S.E., *p < 0.05,**p < 0.01,***p < 0.001). (B) The protein expression patterns of JAK1, STAT3 and apoptosis related genes in zebrafish larvae exposed to 0.5 mg/L cyhalofop-butyl. GAPDH is an internal reference gene. (C) The number of neutrophils in the tail of Tg (lyz: DsRed) transgenic zebrafish treated with ruxolitinib. The white rectangle represents the CHT region in the tail of zebrafish. (D) The statistical chart of neutrophils in Fig. 5C (N ¼ 20, **p < 0.01, ***p < 0.001). CyB: cyhalofop-butyl. Data represent the analysis results of one-way ANOVAeDunnett test. Error bars, mean ± S.E.
4). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]
5). Leptin promotes angiogenesis via pericyte STAT3 pathway upon intracerebral hemorrhage. Cells, 2022 (PubMed: 36078162) [IF=6.0]
6). CTLA4Ig/VISTAIg combination therapy selectively induces CD4+ T cell-mediated immune tolerance by targeting the SOCS1 signaling pathway in porcine islet xenotransplantation. IMMUNOLOGY, 2022 (PubMed: 35263451) [IF=4.9]
7). DACH1 regulates macrophage activation and tumour progression in hypopharyngeal squamous cell carcinoma. Immunology, 2023 (PubMed: 37243970) [IF=4.9]
8). TRIM65 knockout inhibits the development of HCC by polarization tumor-associated macrophages towards M1 phenotype via JAK1/STAT1 signaling pathway. International immunopharmacology, 2024 (PubMed: 38218012) [IF=4.8]
9). Monotropein inhibits colitis associated cancer through VDR/JAK1/STAT1 regulation of macrophage polarization. International Immunopharmacology, 2023 (PubMed: 37633235) [IF=4.8]
10). Silencing of SH3BP2 Inhibits Microglia Activation Via the JAK/STAT Signaling in Spinal Cord Injury Models. Inflammation, 2024 (PubMed: 39546158) [IF=4.5]
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