| 产品: | Slingshot homolog 1 抗体 |
| 货号: | DF13402 |
| 描述: | Rabbit polyclonal antibody to Slingshot homolog 1 |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Bovine, Horse, Sheep, Dog |
| 分子量: | 115kDa; 116kD(Calculated). |
| 蛋白号: | Q8WYL5 |
| RRID: | AB_2846421 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13402, RRID:AB_2846421.
展开/折叠
AW551225; Coro1c; coronin, actin binding protein 1C; Gm1394; Gm1395; hSSH-1L; mSSH 1L; OTTMUSP00000031415; Protein phosphatase Slingshot homolog 1; Slingshot 1; slingshot 1L; slingshot homolog 1; slingshot like 1; Slingshot1; SSH 1; SSH 1L; SSH-1L; SSH-like protein 1; Ssh1; SSH1_HUMAN;
抗原和靶标
- Q8WYL5 SSH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKRSCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGAPEASRERCEDPNAPAICTQPAFLPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGSRRADTSGPGAGAALEPPASLLEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPKKDMKPAKDLRLLFSNESEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPAPPSRDGPASRLEASIPEESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPPFFYRLDHTSSFSKDFLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIETRLRLAGLTVSSPLKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQGTPRDPAATSKPSGKPAPENLKSPSWMSKS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8WYL5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T5 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
| T403 | Phosphorylation | Uniprot | |
| Y407 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T430 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| Y443 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| Y646 | Phosphorylation | Uniprot | |
| K758 | Methylation | Uniprot | |
| S812 | Phosphorylation | Uniprot | |
| T826 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S834 | Phosphorylation | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| S894 | Phosphorylation | Uniprot | |
| S897 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| S910 | Phosphorylation | Uniprot | |
| T922 | Phosphorylation | Uniprot | |
| T933 | Phosphorylation | Uniprot | |
| S935 | Phosphorylation | Uniprot | |
| S936 | Phosphorylation | Uniprot | |
| S937 | Phosphorylation | Q9BZL6 (PRKD2) , Q15139 (PRKD1) , Q13557 (CAMK2D) | Uniprot |
| S938 | Phosphorylation | Uniprot | |
| S940 | Phosphorylation | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| K952 | Acetylation | Uniprot | |
| S970 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S978 | Phosphorylation | Q13557 (CAMK2D) , Q15139 (PRKD1) , Q9BZL6 (PRKD2) | Uniprot |
| T1021 | Phosphorylation | Uniprot | |
| K1041 | Methylation | Uniprot | |
| S1042 | Phosphorylation | Uniprot |
研究背景
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cleavage furrow. Midbody.
Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.
Interacts with actin and this stimulates phosphatase activity. Also interacts with LIMK1 and with the 14-3-3 proteins YWHAB, YWHAG, YWHAQ, and YWHAZ. Interaction with 14-3-3 proteins inhibits phosphatase activity and also blocks recruitment to lamellipodia and stimulation by actin.
Belongs to the protein-tyrosine phosphatase family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
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