Phospho-HDAC5 (Ser498) Antibody - #AF3491

Figure 2. Expression of HDAC4 and HDAC5 in mouse and HepG2 models of WD. Immunoblot densitometry analyses are normalized to β-ACTIN; data are represented as means ± SEM and statistical significance was determined by Student's t-test (*p< 0.05, **p< 0.01, and ****p< 0.0001). A: Total protein liver lysate densitometries for HDAC4 and HDAC5 protein expression in tx-j mice compared to C3H control at post-partum day 6 (PPD6; C3H n=4M/4F, tx-j n=5M/4F), 9 weeks (C3H n=4M/3F, tx-j n=4M/4F), 12 weeks (C3H n=4M/4F, tx-j n=4M/3F), and 24 weeks (C3H n=10M/12F, tx-j n=11M/11F). B: Total protein liver lysate densitometry analyses for HDAC4 and HDAC5 protein expression in 16-week old Atp7b -/- mice (n=7M/4F) and wild-type (WT, n=6M/5F). C: Immunohistochemical analysis of 24-week old C3H and tx-j mouse livers for HDAC5 (red) and DAPI (blue). Images display cytosolic and nuclear HDAC5 localization. White arrows indicate nuclei; scale bar = 50 µm. Bar graphs represent HDAC5 optical density in cytosol, nuclei, and the nucleus/cytosol ratio; n=3 mice/group, 60 cells/mouse. D: HDAC5 immunoblot of HepG2 cell lysates treated with CuSO 4 (0 to 100 µM; n=3 per treatment) for 24 hours. In this figure and all subsequent figures: C3H=C3HeB/FeJ control mice with normal copper metabolism, tx-j=C3He- Atp7b tx-j /J Jackson Laboratory toxic milk mouse model of Wilson disease (WD).

Fig. 2. AKAP5-mediated cardiomyocyte hypertrophy CaMKII/HDAC pathway. (A) Expression of CaMKII/HDAC and hypertrophy-related proteins after H/R and AKAP5 transfection; (B) Expression of CaMKII/HDAC proteins after H/R and siAKAP5 transfection and KN93(10 uM, 1 h) intervention. Analysis by one-way ANOVA, All data are presented as the means ± SD (n = 3). ***P < 0.001 and ****P < 0.0001 vs. the CON group; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the H/R group; **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. the H/R + siAKAP5 group. CON, normal control group; H/R, hypoxia-reoxygenation group; NC, empty vector group; siAKAP5, siRNA-AKAP5 transfection group; KN93, an inhibitor of calmodulin-dependent kinase type II; AKAP5, A-kinase anchoring protein 5; CaMKII, Ca2+/CaM-dependent protein kinase II; HDAC4 /5, Histone deacetylases 4/5; MEF2C, Myocyte enhancer factor 2C.

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05