产品: Endomucin 抗体
货号: DF13357
描述: Rabbit polyclonal antibody to Endomucin
应用: WB IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
蛋白号: Q9ULC0
RRID: AB_2846376

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Endomucin Antibody detects endogenous levels of total Endomucin.
RRID:
AB_2846376
引用格式: Affinity Biosciences Cat# DF13357, RRID:AB_2846376.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EMCN; EMCN2; Endomucin 2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUC14; MUCEN_HUMAN; Mucin 14; Mucin-14;

抗原和靶标

免疫原:

A synthesized peptide derived from human Endomucin, corresponding to a region within C-terminal amino acids.

基因/基因ID:

文献引用

1). Conformationally regulated "nanozyme-like" cerium oxide with multiple free radical scavenging activities for osteoimmunology modulation and vascularized osseointegration. Bioactive materials, 2023 (PubMed: 38186961) [IF=18.9]

Application: IF/ICC    Species: human    Sample: HUVECs

Fig. 4. Angiogenesis of HUVECs in pattern of DSS and CM, respectively. (A) Crystal violet staining images and (B) counting of the invasive HUVECs on Transwell membrane at 24 h. (C) Scratch images at 0 and 12 h and (D) quantified migration length of HUVECs. (E) Vessel tube formation images of HUVECs along with (F) total capillary length and (H) number of branch points per field at 12 h. (G)Fluorescence staining images and relative intensities of (I) CD31 (red) and (J) Emcn (green) in HUVECs at 5 d *p < 0.05.

2). Biomimetic and spatiotemporally sequential hydrogel delivery system with self-healing and adhesion: Triple growth factor for bone defect repair. Chemical Engineering Journal, 2023 [IF=13.3]

3). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2025 (PubMed: 39248296) [IF=12.5]

4). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2024 (PubMed: 39248296) [IF=12.5]

Application: IF/ICC    Species: Rat    Sample:

Figure 7. POL accelerated osteoporotic bone repair by stimulating osteogenesis and angiogenesis via up-regulating β-catenin expression. (A) Images of immunohistochemical staining for VEGFA (scale bar = 200 μm); (B) Quantification of immunohistochemical staining for VEGFA (n = 6); (C) Images of immunohistochemical staining for CD31 (scale bar = 200 μm); (D) Quantification of immunohistochemical staining for CD31 (n = 6); (E) Images of CD31 and EMCN immunofluorescence double-staining (scale bar = 100 μm); (F-H) Quantification of CD31 and EMCN immunofluorescence double-staining (n = 6); (I) Images of immunofluorescence staining for β-catenin (scale bar = 100 μm); (J) Quantification of immunofluorescence staining for β-catenin (n = 6). Data were presented as mean ± SEM. Compared with Sham group: * P < 0.05, **P < 0.01, ***P < 0.001. Compared with OVX + CON group: ### P < 0.001.

5). Chondroitin sulfate/silk fibroin hydrogel incorporating graphene oxide quantum dots with photothermal-effect promotes type H vessel-related wound healing. Carbohydrate polymers, 2024 (PubMed: 38553198) [IF=10.7]

6). Mesenchymal Stem Cell Aggregation-Released Extracellular Vesicles Induce CD31+ EMCN+ Vessels in Skin Regeneration and Improve Diabetic Wound Healing. Advanced healthcare materials, 2023 (PubMed: 36999744) [IF=10.0]

7). A vessel subtype beneficial for osteogenesis enhanced by strontium-doped sodium titanate nanorods by modulating macrophage polarization. Journal of Materials Chemistry B, 2020 (PubMed: 32627795) [IF=6.1]

8). Promoting diabetic oral mucosa wound healing with a light-responsive hydrogel adaptive to the microenvironment. Heliyon, 2024 (PubMed: 39435107) [IF=4.0]

Application: IF/ICC    Species: human    Sample:

Fig. 9 IF staining of diabetic oral mucosa defects across various treatment groups. Scale Bar: 200 μm.

9). Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration. Experimental cell research, 2024 (PubMed: 38768700) [IF=3.3]

10). Soybean Isoflavones Alleviate Osteoarthritis Through Modulation of the TSC1/mTORC1 Signaling Pathway to Reduce Intrachondral Angiogenesis. Immunological investigations, 2024 (PubMed: 39360672) [IF=2.9]

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