Endomucin Antibody - #DF13357

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产品: Endomucin 抗体
货号: DF13357
描述: Rabbit polyclonal antibody to Endomucin
应用: WB IF/ICC
文献验证: IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 27kDa; 27kD(Calculated).
蛋白号: Q9ULC0
RRID: AB_2846376

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MS Report
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WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
Endomucin Antibody detects endogenous levels of total Endomucin.
RRID:
AB_2846376
引用格式: Affinity Biosciences Cat# DF13357, RRID:AB_2846376.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EMCN; EMCN2; Endomucin 2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUC14; MUCEN_HUMAN; Mucin 14; Mucin-14;

抗原和靶标

免疫原:
Uniprot:

Q9ULC0(MUCEN_HUMAN) >>Visit HPA database.

基因/基因ID:
EMCN(51705)
表达:
Q9ULC0 MUCEN_HUMAN:

Expressed in heart, kidney and lung.

序列:
MELLQVTILFLLPSICSSNSTGVLEAANNSLVVTTTKPSITTPNTESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSIISNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTTEIPGSVLQPDASPSKTGTLTSIPVTIPENTSQSQVIGTEGGKNASTSATSRSYSSIILPVVIALIVITLSVFVLVGLYRMCWKADPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

翻译修饰:

Highly O-glycosylated. Sialic acid-rich glycoprotein.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Membrane>Single-pass type I membrane protein.
Note: Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in heart, kidney and lung.

文献引用

1). Conformationally regulated "nanozyme-like" cerium oxide with multiple free radical scavenging activities for osteoimmunology modulation and vascularized osseointegration. Bioactive materials, 2023 (PubMed: 38186961) [IF=18.9]
2). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2025 (PubMed: 39248296) [IF=12.5]
3). Polydatin accelerates osteoporotic bone repair by inducing the osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells via the PI3K/AKT/GSK-3β/β-catenin pathway. International journal of surgery (London, England), 2024 (PubMed: 39248296) [IF=12.5]

Application: IF/ICC    Species: Rat    Sample:

Figure 7. POL accelerated osteoporotic bone repair by stimulating osteogenesis and angiogenesis via up-regulating β-catenin expression. (A) Images of immunohistochemical staining for VEGFA (scale bar = 200 μm); (B) Quantification of immunohistochemical staining for VEGFA (n = 6); (C) Images of immunohistochemical staining for CD31 (scale bar = 200 μm); (D) Quantification of immunohistochemical staining for CD31 (n = 6); (E) Images of CD31 and EMCN immunofluorescence double-staining (scale bar = 100 μm); (F-H) Quantification of CD31 and EMCN immunofluorescence double-staining (n = 6); (I) Images of immunofluorescence staining for β-catenin (scale bar = 100 μm); (J) Quantification of immunofluorescence staining for β-catenin (n = 6). Data were presented as mean ± SEM. Compared with Sham group: * P < 0.05, **P < 0.01, ***P < 0.001. Compared with OVX + CON group: ### P < 0.001.
4). Chondroitin sulfate/silk fibroin hydrogel incorporating graphene oxide quantum dots with photothermal-effect promotes type H vessel-related wound healing. Carbohydrate polymers, 2024 (PubMed: 38553198) [IF=10.7]
5). Mesenchymal Stem Cell Aggregation-Released Extracellular Vesicles Induce CD31+ EMCN+ Vessels in Skin Regeneration and Improve Diabetic Wound Healing. Advanced healthcare materials, 2023 (PubMed: 36999744) [IF=10.0]
6). A vessel subtype beneficial for osteogenesis enhanced by strontium-doped sodium titanate nanorods by modulating macrophage polarization. Journal of Materials Chemistry B, 2020 (PubMed: 32627795) [IF=6.1]
7). Promoting diabetic oral mucosa wound healing with a light-responsive hydrogel adaptive to the microenvironment. Heliyon, 2024 (PubMed: 39435107) [IF=4.0]
8). Effect of M2-macrophage treated lymphatic endothelial cells on angiogenesis that promoted osteointegration. Experimental cell research, 2024 (PubMed: 38768700) [IF=3.3]
9). Soybean Isoflavones Alleviate Osteoarthritis Through Modulation of the TSC1/mTORC1 Signaling Pathway to Reduce Intrachondral Angiogenesis. Immunological investigations, 2024 (PubMed: 39360672) [IF=2.9]
10). Albiflorin improves osteoporotic bone regeneration by promoting osteogenesis-angiogenesis coupling of bone marrow mesenchymal stem cells. Biochemical and biophysical research communications, 2025 (PubMed: 40022815) [IF=2.5]
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