产品: 磷酸化 ALK (Tyr1507) 抗体
货号: AF3489
描述: Rabbit polyclonal antibody to Phospho-ALK (Tyr1507)
应用: WB IHC IF/ICC
反应: Human, Mouse, Monkey
预测: Pig, Zebrafish, Bovine, Rabbit, Dog
分子量: 176kDa; 176kD(Calculated).
蛋白号: Q9UM73
RRID: AB_2834927

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Monkey
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-ALK (Tyr1507) Antibody detects endogenous levels of ALK only when phosphorylated at Tyrosine 1507.
RRID:
AB_2834927
引用格式: Affinity Biosciences Cat# AF3489, RRID:AB_2834927.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alk; ALK tyrosine kinase receptor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; ALK_HUMAN; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; anaplastic lymphoma receptor tyrosine kinase; CD 246; CD246; CD246 antigen; EC 2.7.10.1; Ki 1; Ki1; mutant anaplastic lymphoma kinase; NBLST 3; NBLST3; Tcrz; TFG/ALK;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9UM73 ALK_HUMAN:

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

描述:
a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma.
序列:
MGAIGLLWLLPLLLSTAAVGSGMGTGQRAGSPAAGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARDLLLPPSSSELKAGRPEARGSLALDCAPLLRLLGPAPGVSWTAGSPAPAEARTLSRVLKGGSVRKLRRAKQLVLELGEEAILEGCVGPPGEAAVGLLQFNLSELFSWWIRQGEGRLRIRLMPEKKASEVGREGRLSAAIRASQPRLLFQIFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQMDLLDGPGAERSKEMPRGSFLLLNTSADSKHTILSPWMRSSSEHCTLAVSVHRHLQPSGRYIAQLLPHNEAAREILLMPTPGKHGWTVLQGRIGRPDNPFRVALEYISSGNRSLSAVDFFALKNCSEGTSPGSKMALQSSFTCWNGTVLQLGQACDFHQDCAQGEDESQMCRKLPVGFYCNFEDGFCGWTQGTLSPHTPQWQVRTLKDARFQDHQDHALLLSTTDVPASESATVTSATFPAPIKSSPCELRMSWLIRGVLRGNVSLVLVENKTGKEQGRMVWHVAAYEGLSLWQWMVLPLLDVSDRFWLQMVAWWGQGSRAIVAFDNISISLDCYLTISGEDKILQNTAPKSRNLFERNPNKELKPGENSPRQTPIFDPTVHWLFTTCGASGPHGPTQAQCNNAYQNSNLSVEVGSEGPLKGIQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLEKDDMLYILVGQQGEDACPSTNQLIQKVCIGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPVPLIIAAGGGGRAYGAKTDTFHPERLENNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLQEGATGGHSCPQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGHGEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLAEDGVSCIVSPTPEPHLPLSLILSVVTSALVAALVLAFSGIMIVYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Zebrafish
100
Rabbit
100
Horse
0
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UM73 作为底物

Site PTM Type Enzyme
Y46 Phosphorylation
S47 Phosphorylation
S62 Phosphorylation
S211 Phosphorylation
S312 Phosphorylation
S319 Phosphorylation
S326 Phosphorylation
S329 Phosphorylation
Y705 Phosphorylation
S1075 Phosphorylation
Y1078 Phosphorylation
T1090 Phosphorylation
Y1092 Phosphorylation
Y1096 Phosphorylation
Y1131 Phosphorylation
Y1278 Phosphorylation Q9UM73 (ALK)
Y1282 Phosphorylation Q9UM73 (ALK)
Y1283 Phosphorylation Q9UM73 (ALK)
Y1359 Phosphorylation
Y1401 Phosphorylation
K1451 Methylation
K1455 Methylation
S1481 Phosphorylation
S1495 Phosphorylation
T1506 Phosphorylation
Y1507 Phosphorylation
S1509 Phosphorylation
K1517 Ubiquitination
K1518 Ubiquitination
Y1584 Phosphorylation
Y1586 Phosphorylation
T1597 Phosphorylation
Y1604 Phosphorylation Q9UM73 (ALK)
K1610 Methylation

翻译修饰 - Q9UM73 作为激酶

Substrate Site Source
P14618 (PKM) Y105 Uniprot
P23246 (SFPQ) Y293 Uniprot
P31939 (ATIC) Y104 Uniprot
P40763 (STAT3) Y705 Uniprot
P42768 (WAS) Y102 Uniprot
P49841 (GSK3B) S9 Uniprot
P50552 (VASP) Y39 Uniprot
P62993 (GRB2) Y160 Uniprot
Q06124 (PTPN11) Y546 Uniprot
Q06124 (PTPN11) Y584 Uniprot
Q86WB0 (ZC3HC1) Y105 Uniprot
Q86WB0 (ZC3HC1) Y137 Uniprot
Q9C0H9-5 (SRCIN1) Y396 Uniprot
Q9UM73 (ALK) Y1278 Uniprot
Q9UM73 (ALK) Y1282 Uniprot
Q9UM73 (ALK) Y1283 Uniprot
Q9UM73 (ALK) Y1604 Uniprot

研究背景

功能:

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

翻译修饰:

Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.

N-glycosylated.

细胞定位:

Cell membrane>Single-pass type I membrane protein.
Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

亚基结构:

Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1 (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

文献引用

1). ALK-JNK signaling promotes NLRP3 inflammasome activation and pyroptosis via NEK7 during Streptococcus pneumoniae infection. Molecular Immunology (PubMed: 37001294) [IF=3.6]

Application: WB    Species: Mouse    Sample:

Fig. 3. Phosphorylated-ALK is involved in the S. pneumoniae infection-induced NLRP3 inflammasome activation. A-B: PECs were infected with S. pneumoniae at an MOI of 5 for 0, 5, 15, 30 or 60 min. A: The cell lysates were collected, and total and phosphorylation levels of ALK were detected by western blot. B: P-ALK/t-ALK was analyzed by relative densitometric quantification. C-J: PECs were pretreated with or without ALK inhibitor (Bri, 20nmol/L) for 2 h and then infected with S. pneumoniae (MOI of 5) for 6 h. C-F: The protein expression levels of pro-caspase-1, p20, pro-IL-1β and p17 in supernatants or cell lysates were detected by western blot and analyzed by relative densitometric quantification. G: The mRNA expression levels of Caspase-1 and Il-1β were quantified by RT-PCR. H: The protein expression level of NLRP3 were detected by western blot and analyzed by relative densitometric quantification. I: The mRNA expression level of Nlrp3 was detected by RT-PCR. J: The ASC specks formation were imaged via fluorescence microscopy. (Scale bar: 100 µm.) K: PECs were pretreated with or without Bri for 2 h and then infected with S. pneumoniae (MOI of 1) for 24 h.The supernatants were collected and the level of IL-1β in supernatants were detected by ELISA. L-N: PECs were pretreated with or without Bri for 2 h and then infected with S. pneumoniae (MOI of 5) for 6 h. L: The protein expression level of NEK7 were detected by western blot and analyzed by relative densitometric quantification. M: The mRNA expression level of Nek7 was detected by RT-PCR. N: The NEK7-NLRP3 complex formation were detected by Co-IP. Similar results were obtained in three independent experiments in data. Data shown are means ± SEMs; *P 

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