S100 A1 Antibody - #AF0251

>>买2送1 进行中>>
(3)   (9)  
产品: S100 A1 抗体
货号: AF0251
描述: Rabbit polyclonal antibody to S100 A1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 10kDa; 11kD(Calculated).
蛋白号: P23297
RRID: AB_2833426

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(85%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(93%)
克隆:
Polyclonal
特异性:
S100 A1 Antibody detects endogenous levels of total S100 A1.
RRID:
AB_2833426
引用格式: Affinity Biosciences Cat# AF0251, RRID:AB_2833426.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Bpb; NEF; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 Alpha Chain; S100; S100 Beta Chain; S100 Calcium Binding Protein A1; S100 Calcium Binding Protein B; S100 Calcium Binding Protein Beta Neural; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100A; s100a1; S10A1_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P23297(S10A1_HUMAN) >>Visit HPA database.

基因/基因ID:
S100A1(6271)
表达:
P23297 S10A1_HUMAN:

Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

描述:
S100A1 Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
序列:
MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
93
Zebrafish
85
Xenopus
71
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23297 作为底物

Site PTM Type Enzyme
Y27 Phosphorylation
S43 Phosphorylation
C86 S-Nitrosylation

研究背景

功能:

Probably acts as a Ca(2+) signal transducer. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers a conformational change. This conformational change allows interaction of S1001A with specific target proteins, such as TPR-containing proteins, and the modulation of their activity.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

亚基结构:

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Also forms heterodimers with S100P. Interacts with AGER (By similarity). Interacts with CAPZA1 (By similarity). Interacts with FKBP4. Interacts with RYR1 and RYR2. Interacts with CACYBP in a calcium-dependent manner. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.

蛋白家族:

Belongs to the S-100 family.

文献引用

1). The effects of exosomes originating from different cell sources on the differentiation of bone marrow mesenchymal stem cells into schwann cells. Journal of nanobiotechnology, 2024 (PubMed: 38698449) [IF=10.2]

Application: WB    Species: Rat    Sample: BMSCs

Fig. 2 Protein expression of Schwann cell markers in differentiallyinduced BMSCs(n = 3). (A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm. (B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groups

Application: IF/ICC    Species: Rat    Sample: BMSCs

Fig. 2 Protein expression of Schwann cell markers in differentiallyinduced BMSCs(n = 3). (A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm. (B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groups
2). Schwann cell‑derived exosomes induce bone marrow‑derived mesenchymal stem cells to express Schwann cell markers in vitro. Molecular Medicine Reports, 2020 (PubMed: 32016464) [IF=3.4]

Application: WB    Species: rat    Sample: BMSCs

Figure 3. |mRNA and protein expression levels of Schwann cell markers in differentially‑induced BMSCs. (F and G) Representative immunoblot of protein expression levels of S100, GFAP, NGRF, Sox10, EGR2 and GAPDH in control BMSCs, induced BMSCs, BMSCs + RSC96 exo, BMSCs + Fb exo or RSC96 cells. Data are presented as mean ± SD; *P<0.05, **P<0.01 and ***P<0.001. BMSCs, bone marrow mesenchymal stem cells; EGR2, early growth response 2; Fb exo, fibroblast‑derived exosomes; GFAP, glial fibrillary acidic protein; induced BMSCs, Dezawa's induction method; NGFR, low‑affinity nerve growth factor receptor; RSC96 exo, Schwann cell‑derived exosomes.
3). Effect of Exosomes from Different Cell Sources on Differentiation of Bone Marrow Mesenchymal Stem Cells into Schwann Cells.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.