PDE4A Antibody - #DF13317
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13317, RRID:AB_2846336.
展开/折叠
5''-cyclic phosphodiesterase 4A; cAMP specific phosphodiesterase; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; DPDE2; dunce like phosphodiesterase E2; PDE 4A; PDE4; PDE46; PDE4A; PDE4A_HUMAN; PDE4A11; Phosphodiesterase 4A; phosphodiesterase 4A cAMP specific; Phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog; Phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; phosphodiesterase type 4A11;
抗原和靶标
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
- P27815 PDE4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPTVPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERAERERQPHRPIERADAMDTSDRPGLRTTRMSWPSSFHGTGTGSGGAGGGSSRRFEAENGPTPSPGRSPLDSQASPGLVLHAGAATSQRRESFLYRSDSDYDMSPKTMSRNSSVTSEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPVPSNKRSPLGGPTPVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
翻译修饰 - P27815 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S152 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| S157 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K358 | Sumoylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| T551 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| T870 | Phosphorylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| T886 | Phosphorylation | Uniprot |
研究背景
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
Proteolytically cleaved by caspase-3.
Cytoplasm>Perinuclear region.
Cytoplasm>Perinuclear region. Cell projection>Ruffle membrane.
Membrane>Peripheral membrane protein.
Note: Isoform 4 has propensity for association with membranes.
Cytoplasm>Perinuclear region.
Cytoplasm. Membrane.
Note: Predominantly cytoplasmic.
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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