产品: 磷酸化 HDAC6 (Ser22) 抗体
货号: AF3485
描述: Rabbit polyclonal antibody to Phospho-HDAC6 (Ser22)
应用: WB IHC IF/ICC
反应: Human, Mouse
分子量: 131kDa; 131kD(Calculated).
蛋白号: Q9UBN7
RRID: AB_2834923

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
Phospho-HDAC6 (Ser22) Antibody detects endogenous levels of HDAC6 only when phosphorylated at Serine 22.
RRID:
AB_2834923
引用格式: Affinity Biosciences Cat# AF3485, RRID:AB_2834923.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CPBHM; FLJ16239; HD 6; HD6; HDAC 6; HDAC6; HDAC6_HUMAN; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM 21; JM21; KIAA0901; OTTHUMP00000032398; OTTHUMP00000197663; PPP1R90; Protein phosphatase 1 regulatory subunit 90;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
序列:
MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH

翻译修饰 - Q9UBN7 作为底物

Site PTM Type Enzyme
S16 Phosphorylation
S22 Phosphorylation P49841 (GSK3B)
S28 Phosphorylation
T30 Phosphorylation
K32 Ubiquitination
K36 Acetylation
K37 Acetylation
S43 Phosphorylation
K51 Ubiquitination
K116 Ubiquitination
S412 Phosphorylation
S458 Phosphorylation P68400 (CSNK2A1)
Y570 Phosphorylation P00533 (EGFR)
S846 Phosphorylation
S847 Phosphorylation
K849 Acetylation
K849 Ubiquitination
K853 Acetylation
K854 Acetylation
K854 Ubiquitination
K860 Ubiquitination
T868 Phosphorylation
K873 Acetylation
K881 Acetylation
T923 Phosphorylation
S925 Phosphorylation
T993 Phosphorylation
S995 Phosphorylation
T1003 Phosphorylation
T1016 Phosphorylation
T1021 Phosphorylation
T1027 Phosphorylation
T1031 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
T1034 Phosphorylation
S1035 Phosphorylation P27361 (MAPK3)
T1040 Phosphorylation
T1041 Phosphorylation
T1048 Phosphorylation
T1055 Phosphorylation
S1062 Phosphorylation
K1206 Ubiquitination

研究背景

功能:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.

翻译修饰:

Phosphorylated by AURKA.

Ubiquitinated. Its polyubiquitination however does not lead to its degradation.

Sumoylated in vitro.

细胞定位:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Perikaryon. Cell projection>Dendrite. Cell projection>Axon.
Note: It is mainly cytoplasmic, where it is associated with microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with SIRT2 (via both phosphorylated, unphosphorylated, active or inactive forms); the interaction is necessary for the complex to interact with alpha-tubulin. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with BBIP10, CBFA2T3, CYLD, DDIT3/CHOP, ZMYND15, F-actin and HDAC11. Interacts with RIPOR2; this interaction occurs during early myogenic differentiation and prevents HDAC6 to deacetylate tubulin. Interacts with DYSF; this interaction occurs during early myogenic differentiation. Interacts with TPPP; inhibiting the tubulin deacetylase activity of HDAC6. Interacts with DYNLL1.

蛋白家族:

Belongs to the histone deacetylase family. HD type 2 subfamily.

研究领域

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Chen Y et al. OCT4B-190 protects against ischemic stroke by modulating GSK-3β/HDAC6. EXPERIMENTAL NEUROLOGY 2019 Jun;316:52-62 (PubMed: 30981804) [IF=5.3]

Application: WB    Species: mouse    Sample: primary neuronal cultures

Supplementary Fig. 7. |The independence of pHDAC6 with GSK-3β kinase activation. Primary cortical neurons were infected with adenovirus expressing OCT4B-190 or control adenovirus for 3 days, and then neurons were subjected to DMSO or AKT inhibitor (AKT-I), 1 h after which the expression of GSK-3β and HDAC6 was examined by western blotting. A. Representative immunoblot of GSK-3βand HDAC6 from primary neuronal cultures.

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