CHD4 Antibody - #DF13295

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产品: CHD4 抗体
货号: DF13295
描述: Rabbit polyclonal antibody to CHD4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 218kDa; 218kD(Calculated).
蛋白号: Q14839
RRID: AB_2846314

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
CHD4 Antibody detects endogenous levels of total CHD4.
RRID:
AB_2846314
引用格式: Affinity Biosciences Cat# DF13295, RRID:AB_2846314.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP dependent helicase CHD4; ATP-dependent helicase CHD4; CHD 4; CHD-4; CHD4; CHD4_HUMAN; Chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi-2 autoantigen 218 kDa protein; Mi2 beta; Mi2-beta;

抗原和靶标

免疫原:
Uniprot:

Q14839(CHD4_HUMAN) >>Visit HPA database.

基因/基因ID:
CHD4(1108)
序列:
MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPRDPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDGYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQDETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDGSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQQVAQQQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14839 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
S27 Phosphorylation
S44 Phosphorylation
T46 Phosphorylation
S85 Phosphorylation
S86 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
S108 Phosphorylation
Y110 Phosphorylation
K115 Acetylation
K116 Acetylation
K118 Acetylation
K119 Acetylation
K123 Acetylation
S150 Phosphorylation
S151 Phosphorylation
S168 Phosphorylation
K179 Ubiquitination
K195 Ubiquitination
K273 Acetylation
K275 Acetylation
S277 Phosphorylation
K285 Acetylation
K297 Ubiquitination
S303 Phosphorylation
K304 Acetylation
K304 Ubiquitination
S308 Phosphorylation
S309 Phosphorylation
S310 Phosphorylation
S319 Phosphorylation
S325 Phosphorylation
T360 Phosphorylation
Y365 Phosphorylation
T367 Phosphorylation
Y372 Phosphorylation
S428 Phosphorylation
K499 Ubiquitination
K504 Ubiquitination
S515 Phosphorylation
T517 Phosphorylation
T529 Phosphorylation P24941 (CDK2)
S531 Phosphorylation P24941 (CDK2)
K533 Ubiquitination
S585 Phosphorylation
K594 Ubiquitination
K604 Ubiquitination
K618 Sumoylation
K618 Ubiquitination
S631 Phosphorylation
K634 Ubiquitination
K635 Ubiquitination
K668 Ubiquitination
R679 Methylation
K696 Ubiquitination
T703 Phosphorylation
T705 Phosphorylation
T709 Phosphorylation
K711 Sumoylation
K711 Ubiquitination
K832 Ubiquitination
K884 Acetylation
K884 Ubiquitination
K959 Ubiquitination
K964 Ubiquitination
S979 Phosphorylation
K987 Acetylation
K987 Ubiquitination
R1000 Methylation
S1007 Phosphorylation
K1016 Acetylation
K1016 Ubiquitination
K1017 Acetylation
K1017 Ubiquitination
K1051 Ubiquitination
K1057 Ubiquitination
S1073 Phosphorylation
K1092 Ubiquitination
K1206 Ubiquitination
T1207 Phosphorylation
S1209 Phosphorylation
K1212 Ubiquitination
K1220 Ubiquitination
K1228 Sumoylation
K1228 Ubiquitination
S1244 Phosphorylation
S1245 Phosphorylation
K1252 Ubiquitination
T1265 Phosphorylation
Y1276 Phosphorylation
K1281 Ubiquitination
R1288 Methylation
K1304 Sumoylation
K1304 Ubiquitination
S1308 Phosphorylation
Y1313 Phosphorylation
K1316 Ubiquitination
R1319 Methylation
K1335 Ubiquitination
S1349 Phosphorylation Q13315 (ATM)
S1370 Phosphorylation
K1390 Ubiquitination
K1396 Ubiquitination
K1398 Sumoylation
K1398 Ubiquitination
K1422 Ubiquitination
S1500 Phosphorylation
K1505 Ubiquitination
K1528 Ubiquitination
K1529 Sumoylation
S1531 Phosphorylation
S1535 Phosphorylation
S1537 Phosphorylation
T1540 Phosphorylation
T1542 Phosphorylation
S1544 Phosphorylation
T1545 Phosphorylation
T1549 Phosphorylation
T1553 Phosphorylation
K1565 Sumoylation
S1570 Phosphorylation
K1572 Sumoylation
K1572 Ubiquitination
S1576 Phosphorylation
K1584 Ubiquitination
C1594 S-Nitrosylation
T1595 Phosphorylation
S1602 Phosphorylation
K1643 Acetylation
K1643 Ubiquitination
K1647 Sumoylation
K1647 Ubiquitination
S1648 Phosphorylation
T1653 Phosphorylation
K1660 Ubiquitination
K1670 Sumoylation
T1679 Phosphorylation
T1722 Phosphorylation
K1724 Ubiquitination
T1725 Phosphorylation
K1766 Ubiquitination
Y1804 Phosphorylation
S1808 Phosphorylation
C1827 S-Nitrosylation
S1831 Phosphorylation
K1837 Ubiquitination
S1839 Phosphorylation
K1844 Ubiquitination
K1852 Ubiquitination
K1855 Ubiquitination
K1865 Ubiquitination
S1888 Phosphorylation
T1904 Phosphorylation

研究背景

功能:

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

细胞定位:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Associates with centrosomes in interphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with KLF1; the interaction depends on sumoylation of KLF1, and leads to its transcriptional repression (By similarity). Interacts directly with IKFZ1 in the NuRD complex. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Part of a complex containing ATR and HDAC2. Interacts with TRIM27. Interacts with ZGPAT; the interaction is direct. Interacts with BCL6. Interacts with BRD4. Interacts with PCNT. Interacts with SETX. Interacts with HDAC1.

蛋白家族:

Belongs to the SNF2/RAD54 helicase family.

研究领域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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