CD36 Antibody - #DF13262

Figure 6. | Uptake of CAF-derived lipids by CRC through increased membrane fluidity.F, Western blot showing CD36 expression in DLD1 cells incubated with CAF-CM.

FIGURE 7 APOC2 cooperates with CD36 to promote tumor progression and PM in GC. (A) Flag‐APOC2 interacts with His‐CD36 in AGS and BGC‐823 cells. The cells were cotransfected with Flag‐APOC2 and His‐CD36. For the co‐IP assays, anti‐FLAG antibody was used for pulldown and anti‐His antibody was detected by western blot. (B) Measurement of the binding affinity of APOC2 peptide to CD36 by biolayer interferometry method. Various concentrations of APOC2 peptide were shown. (C–E) The effect of knocking down CD36 on the migration, invasion, and proliferation of OE APOC2 stable cells. (F) The levels of p‐PI3K, p‐AKT, p‐mTOR, E cadherin, N‐cadherin, vimentin, Snail, Slug, Twist1, MMP‐2, and MMP‐9 proteins detected by western blot in OE APOC2 AGS cells transduced with shCD36 lentiviral particles. (G–I) Representative images of the macroscopic appearance and hematoxylin and eosin (H&E) staining of PM nodules in nude mice treated with intraperitoneal injection of AGS OE APOC2/shCtrol, AGS OE APOC2/shCD36, AGS OE CD36/shCtrol, and AGS OE CD36/shAPOC2 cells, respectively (n = 6 per group). The total number (H) and weight (I) of PM nodules in the respective groups. Data are shown as mean ± SD; ns, no significant difference; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, based on Student's t‐test

Fig. 6. The effect of QSYQ on the expression of CD36, PPARγ, LXRα/β, and ABCA1 in atherosclerotic plaque. (A) Representative pictures of CD36, PPARγ, LXRα/β, and ABCA1 immunohistochemical staining in the aortic root of each group. Scale bar = 100 μm. (B) The positive staining area and plaque area were measured by image J analysis software, and the ratio of the positive staining area and plaque area was calculated. ★, P＜0.05, ★★, P＜0.01, considered as significant difference. n.s., P＞0.05, considered as no difference.