产品: 磷酸化 p62 Dok (Tyr362) 抗体
货号: AF3478
描述: Rabbit polyclonal antibody to Phospho-p62 Dok (Tyr362)
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 62kDa; 52kD(Calculated).
蛋白号: Q99704
RRID: AB_2834916

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-p62 Dok (Tyr362) Antibody detects endogenous levels of p62 Dok only when phosphorylated at Tyrosine 362.
RRID:
AB_2834916
引用格式: Affinity Biosciences Cat# AF3478, RRID:AB_2834916.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q99704 DOK1_HUMAN:

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

描述:
Dok1 a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase.
序列:
MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
78
Chicken
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99704 作为底物

Site PTM Type Enzyme
M1 Acetylation
Y30 Phosphorylation
S33 Phosphorylation
R39 Methylation
K46 Ubiquitination
S48 Phosphorylation
T93 Phosphorylation
K121 Ubiquitination
S123 Phosphorylation
T125 Phosphorylation
Y146 Phosphorylation
Y203 Phosphorylation
S217 Phosphorylation
K257 Ubiquitination
S269 Phosphorylation
S281 Phosphorylation
S291 Phosphorylation
Y296 Phosphorylation
C308 S-Nitrosylation
S310 Phosphorylation
S313 Phosphorylation
Y315 Phosphorylation
S316 Phosphorylation
Y337 Phosphorylation
Y341 Phosphorylation
T354 Phosphorylation
Y362 Phosphorylation P00519 (ABL1) , P06213 (INSR) , Q13882 (PTK6)
Y377 Phosphorylation
K394 Ubiquitination
Y398 Phosphorylation P07949 (RET) , P06213 (INSR)
Y402 Phosphorylation
T406 Phosphorylation
Y409 Phosphorylation
T435 Phosphorylation
S439 Phosphorylation O14920 (IKBKB)
S443 Phosphorylation O14920 (IKBKB)
S446 Phosphorylation O14920 (IKBKB)
Y449 Phosphorylation
S450 Phosphorylation O14920 (IKBKB)
S458 Phosphorylation
S460 Phosphorylation
T470 Phosphorylation
T481 Phosphorylation

研究背景

功能:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

翻译修饰:

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.

细胞定位:

Cytoplasm. Nucleus.

Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.

亚基结构:

Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Interacts with SRMS (via the SH2 and SH3 domains).

蛋白家族:

The PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

研究领域

· Human Diseases > Infectious diseases: Viral > Measles.

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