Stra8 Antibody - #DF13234

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice. (a) miR-34c-5p levels in testicular tissue (n = 6); (b) representative Western blots using antibodies against RARg, Stra8, and PLZF in testicular tissue (n = 6). (c–e) The bar graphs show the results of the semi-quantitative measurement of RARg, Stra8, and PLZF expression, respectively. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, versus DIO group.

Fig. 3. Spermatogonial population dynamics during neonatal development are altered in the absence of PRAMEL1. (A) Immunofluorescent staining with PLZF (green) and STRA8 (red) antibodies, counterstained with DAPI (blue), on testis cross-sections at P2-P6. Arrows indicate the PLZF+/STRA8+ double-positive cells. Negative controls are in the right top corners of images. (B) Number of STRA8+/PLZF+ double-positive spermatogonia/mm2 and of PLZF+ cells/mm2. (C) Relative RNA expression level (wild type is set as 1, dashed line) in spermatogonia. (D) Flow cytometry analysis of ID4-EGFPBright, ID4-EGFPMid, ID4-EGFPDim and ID4-EGFP− among DDX4+ cells. The immunofluorescent staining experiments in A, qRT-PCR in C and flow cytometry analysis in D were repeated three times. Data are mean±s.e.m., n=4 or 5; *P