Stra8 Antibody - #DF13234

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产品: Stra8 抗体
货号: DF13234
描述: Rabbit polyclonal antibody to Stra8
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
蛋白号: Q7Z7C7
RRID: AB_2846253

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Stra8 Antibody detects endogenous levels of total Stra8.
RRID:
AB_2846253
引用格式: Affinity Biosciences Cat# DF13234, RRID:AB_2846253.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Stimulated by retinoic acid 8; stimulated by retinoic acid 8 homolog; Stimulated by retinoic acid Gene 8; stimulated by retinoic acid gene 8 homolog; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8; STRA8_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Stra8, corresponding to a region within the internal amino acids.

基因/基因ID:
STRA8(346673)

文献引用

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=5.9]

Application: WB    Species: Rat    Sample: spermatogenic cell

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p‐Akt, caspase‐9, Bcl‐2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3. (B) Statistical analysis of band intensity by Student's t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. NS, not significant; Sham, sham group; Surgical treatment, surgical treatment group; VC, varicocele group

Application: WB    Species: rat    Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.
2). Differential Impairment Mechanism of Sperm Production via Induction of miR-34c-Activated Apoptosis and Spermatogenesis Pathway in Diet-Induced Obesity and Resistant Mice and GC-1 Spg Cells. International journal of molecular sciences, 2024 (PubMed: 39000558) [IF=5.6]

Application: WB    Species: Mouse    Sample: testicular tissues

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice. (a) miR-34c-5p levels in testicular tissue (n = 6); (b) representative Western blots using antibodies against RARg, Stra8, and PLZF in testicular tissue (n = 6). (c–e) The bar graphs show the results of the semi-quantitative measurement of RARg, Stra8, and PLZF expression, respectively. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, versus DIO group.
3). Mouse Pramel1 regulates spermatogonial development by inhibiting retinoic acid signaling during spermatogenesis. Development (Cambridge, England), 2023 (PubMed: 37781892) [IF=3.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 3. Spermatogonial population dynamics during neonatal development are altered in the absence of PRAMEL1. (A) Immunofluorescent staining with PLZF (green) and STRA8 (red) antibodies, counterstained with DAPI (blue), on testis cross-sections at P2-P6. Arrows indicate the PLZF+/STRA8+ double-positive cells. Negative controls are in the right top corners of images. (B) Number of STRA8+/PLZF+ double-positive spermatogonia/mm2 and of PLZF+ cells/mm2. (C) Relative RNA expression level (wild type is set as 1, dashed line) in spermatogonia. (D) Flow cytometry analysis of ID4-EGFPBright, ID4-EGFPMid, ID4-EGFPDim and ID4-EGFP− among DDX4+ cells. The immunofluorescent staining experiments in A, qRT-PCR in C and flow cytometry analysis in D were repeated three times. Data are mean±s.e.m., n=4 or 5; *P

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