Stra8 Antibody - #DF13234

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产品: Stra8 抗体
货号: DF13234
描述: Rabbit polyclonal antibody to Stra8
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 36 kd; 37kD(Calculated).
蛋白号: Q7Z7C7
RRID: AB_2846253

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Dog(%)
克隆:
Polyclonal
特异性:
Stra8 Antibody detects endogenous levels of total Stra8.
RRID:
AB_2846253
引用格式: Affinity Biosciences Cat# DF13234, RRID:AB_2846253.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Stimulated by retinoic acid 8; stimulated by retinoic acid 8 homolog; Stimulated by retinoic acid Gene 8; stimulated by retinoic acid gene 8 homolog; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8; STRA8_HUMAN;

抗原和靶标

免疫原:
Uniprot:

Q7Z7C7(STRA8_HUMAN) >>Visit HPA database.

基因/基因ID:
STRA8(346673)
表达:
Q7Z7C7 STRA8_HUMAN:

Expressed specifically in testis and fetal ovaries.

序列:
MGKIDVDKILFFNQEIRLWQLIMATPEENSNPHDRATPQLPAQLQELEHRVARRRLSQARHRATLAALFNNLRKTVYSQSDLIASKWQVLNKAKSHIPELEQTLDNLLKLKASFNLEDGHASSLEEVKKEYASMYSGNDSFPQNGSSPWYLNFYKQTMDLLTGSGIITPQEAALPIVSAAISHLWQNLSEERKASLRQAWAQKHRGPATLAEACREPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEVPSTSSSSSVLASCNPENPEEKFQLYMQIINFFKGLSCANTQVKQEASFPVDEEMIMLQCTETFDDEDL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription (By similarity).

翻译修饰:

Phosphorylated.

细胞定位:

Cytoplasm. Nucleus.
Note: Shuttles between nucleus and cytoplasm. Nuclear export is XPO1-dependent.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed specifically in testis and fetal ovaries.

亚基结构:

Interacts with XPO1.

文献引用

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=5.9]

Application: WB    Species: Rat    Sample: spermatogenic cell

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p‐Akt, caspase‐9, Bcl‐2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3. (B) Statistical analysis of band intensity by Student's t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. NS, not significant; Sham, sham group; Surgical treatment, surgical treatment group; VC, varicocele group

Application: WB    Species: rat    Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.
2). Differential Impairment Mechanism of Sperm Production via Induction of miR-34c-Activated Apoptosis and Spermatogenesis Pathway in Diet-Induced Obesity and Resistant Mice and GC-1 Spg Cells. International journal of molecular sciences, 2024 (PubMed: 39000558) [IF=5.6]

Application: WB    Species: Mouse    Sample: testicular tissues

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice. (a) miR-34c-5p levels in testicular tissue (n = 6); (b) representative Western blots using antibodies against RARg, Stra8, and PLZF in testicular tissue (n = 6). (c–e) The bar graphs show the results of the semi-quantitative measurement of RARg, Stra8, and PLZF expression, respectively. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, versus DIO group.

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