Furin Antibody - #DF13231

Figure 1 Tumor cell GA-targeting self-assembled peptide induces GA dysfunction and cell apoptosis. A) Furin expression in L929 and 4T1 cells. B) Quantitative analysis of furin in L929 and 4T1 cells by ELISA. C) CLSM images of co-localization of furin with GA in 4T1 cells. D) Schematic diagram of the chemical structure and assembly of the functional targeting peptide NF-1 and its two control peptides (NF-2 and NF-3) in aqueous solution. Different functional units of amino acid sequences were shown in various colors. Blue represents the tumor cell-targeting motif; laurel green represents the GA-targeting and furin-responsive cleavage motif; pink represents the motif of being capable of forming fiber, and yellow represents the motif of being unable to form fiber. E) Representative TEM images of self-assembly of NF-1, NF-2, and NF-3 after incubation with recombinant furin (0.5 µg mL−1) at different times. F) HPLC analysis of NF-1, NF-1 with recombinant furin (0.5 µg mL−1), and NF-3 for 12 h. G) Representative CLSM images of co-localization of NF-1RhB (2 µm) with Golgi-Tracker green in 4T1 cells after co-culture for 8 h. Nucleus, blue (DAPI). H) Pearson's coefficient of CLSM images between NF-1RhB (2 µm) and different cell organelles, including the nucleus, endoplasmic reticulum, lysosome, mitochondria, and GA in 4T1 cells after 8 h of co-culture. I) Representative TEM images of 4T1 cells incubated with PBS, NF-1 (100 µm), and NF-2 (100 µm) for 24 h, respectively. J) Immunofluorescence images of GM130 after PBS, NF-1, or NF-2 treatment in 4T1 cells. K) RUSH system analysis of the protein process and transportation in 4T1 cells after incubation of NF-1 (100 µm) and NF-2 (100 µm) for 24 h, respectively. L) Cell viability curves of 4T1 cells that were incubated with different peptides (NF-1, NF-2) at various concentrations (0, 20, 40, 80, 100, 200, 400, and 600 µm) for 48 h. M) Statistical results of apoptosis assay of 4T1 cells after incubation with PBS, NF-1 (100 µm), and NF-2 (100 µm) for 24 h. N) Diagram of GA dysfunction and apoptosis of tumor cells induced by tumor cell GA-targeting self-assembled peptide.