Pericentrin - Centrosome Marker Antibody - #DF13210

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产品: Pericentrin - Centrosome Marker 抗体
货号: DF13210
描述: Rabbit polyclonal antibody to Pericentrin - Centrosome Marker
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 378kDa; 378kD(Calculated).
蛋白号: O95613
RRID: AB_2846229

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Bovine(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
Pericentrin - Centrosome Marker Antibody detects endogenous levels of total Pericentrin - Centrosome Marker.
RRID:
AB_2846229
引用格式: Affinity Biosciences Cat# DF13210, RRID:AB_2846229.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Centrosome Marker; Ken; Kendrin; KIAA0402; MOPD2; PCN; PCNT 2; PCNT; PCNT B; PCNT_HUMAN; PCNT1; PCNT2; PCNTB; PCTN2; Pericentrin 1; Pericentrin 2; Pericentrin 380; Pericentrin; Pericentrin B; Pericentrin-B; SCKL4;

抗原和靶标

免疫原:
Uniprot:

O95613(PCNT_HUMAN) >>Visit HPA database.

基因/基因ID:
PCNT(5116)
表达:
O95613 PCNT_HUMAN:

Expressed in all tissues tested, including placenta, liver, kidney and thymus.

序列:
MEVEQEQRRRKVEAGRTKLAHFRQRKTKGDSSHSEKKTAKRKGSAVDASVQEESPVTKEDSALCGGGDICKSTSCDDTPDGAGGAFAAQPEDCDGEKREDLEQLQQKQVNDHPPEQCGMFTVSDHPPEQHGMFTVGDHPPEQRGMFTVSDHPPEQHGMFTVSDHPPEQRGMFTISDHQPEQRGMFTVSDHTPEQRGIFTISDHPAEQRGMFTKECEQECELAITDLESGREDEAGLHQSQAVHGLELEALRLSLSNMHTAQLELTQANLQKEKETALTELREMLNSRRAQELALLQSRQQHELELLREQHAREKEEVVLRCGQEAAELKEKLQSEMEKNAQIVKTLKEDWESEKDLCLENLRKELSAKHQSEMEDLQNQFQKELAEQRAELEKIFQDKNQAERALRNLESHHQAAIEKLREDLQSEHGRCLEDLEFKFKESEKEKQLELENLQASYEDLKAQSQEEIRRLWSQLDSARTSRQELSELHEQLLARTSRVEDLEQLKQREKTQHESELEQLRIYFEKKLRDAEKTYQEDLTLLQQRLQGAREDALLDSVEVGLSCVGLEEKPEKGRKDHVDELEPERHKESLPRFQAELEESHRHQLEALESPLCIQHEGHVSDRCCVETSALGHEWRLEPSEGHSQELPWVHLQGVQDGDLEADTERAARVLGLETEHKVQLSLLQTELKEEIELLKIENRNLYGKLQHETRLKDDLEKVKHNLIEDHQKELNNAKQKTELMKQEFQRKETDWKVMKEELQREAEEKLTLMLLELREKAESEKQTIINKFELREAEMRQLQDQQAAQILDLERSLTEQQGRLQQLEQDLTSDDALHCSQCGREPPTAQDGELAALHVKEDCALQLMLARSRFLEERKEITEKFSAEQDAFLQEAQEQHARELQLLQERHQQQLLSVTAELEARHQAALGELTASLESKQGALLAARVAELQTKHAADLGALETRHLSSLDSLESCYLSEFQTIREEHRQALELLRADFEEQLWKKDSLHQTILTQELEKLKRKHEGELQSVRDHLRTEVSTELAGTVAHELQGVHQGEFGSEKKTALHEKEETLRLQSAQAQPFHQEEKESLSLQLQKKNHQVQQLKDQVLSLSHEIEECRSELEVLQQRRERENREGANLLSMLKADVNLSHSERGALQDALRRLLGLFGETLRAAVTLRSRIGERVGLCLDDAGAGLALSTAPALEETWSDVALPELDRTLSECAEMSSVAEISSHMRESFLMSPESVRECEQPIRRVFQSLSLAVDGLMEMALDSSRQLEEARQIHSRFEKEFSFKNEETAQVVRKHQELLECLKEESAAKAELALELHKTQGTLEGFKVETADLKEVLAGKEDSEHRLVLELESLRRQLQQAAQEQAALREECTRLWSRGEATATDAEAREAALRKEVEDLTKEQSETRKQAEKDRSALLSQMKILESELEEQLSQHRGCAKQAEAVTALEQQVASLDKHLRNQRQFMDEQAAEREHEREEFQQEIQRLEGQLRQAAKPQPWGPRDSQQAPLDGEVELLQQKLREKLDEFNELAIQKESADRQVLMQEEEIKRLEEMNINIRKKVAQLQEEVEKQKNIVKGLEQDKEVLKKQQMSSLLLASTLQSTLDAGRCPEPPSGSPPEGPEIQLEVTQRALLRRESEVLDLKEQLEKMKGDLESKNEEILHLNLKLDMQNSQTAVSLRELEEENTSLKVIYTRSSEIEELKATIENLQENQKRLQKEKAEEIEQLHEVIEKLQHELSLMGPVVHEVSDSQAGSLQSELLCSQAGGPRGQALQGELEAALEAKEALSRLLADQERRHSQALEALQQRLQGAEEAAELQLAELERNVALREAEVEDMASRIQEFEAALKAKEATIAERNLEIDALNQRKAAHSAELEAVLLALARIRRALEQQPLAAGAAPPELQWLRAQCARLSRQLQVLHQRFLRCQVELDRRQARRATAHTRVPGAHPQPRMDGGAKAQVTGDVEASHDAALEPVVPDPQGDLQPVLVTLKDAPLCKQEGVMSVLTVCQRQLQSELLLVKNEMRLSLEDGGKGKEKVLEDCQLPKVDLVAQVKQLQEKLNRLLYSMTFQNVDAADTKSLWPMASAHLLESSWSDDSCDGEEPDISPHIDTCDANTATGGVTDVIKNQAIDACDANTTPGGVTDVIKNWDSLIPDEMPDSPIQEKSECQDMSLSSPTSVLGGSRHQSHTAEAGPRKSPVGMLDLSSWSSPEVLRKDWTLEPWPSLPVTPHSGALSLCSADTSLGDRADTSLPQTQGPGLLCSPGVSAAALALQWAESPPADDHHVQRTAVEKDVEDFITTSFDSQETLSSPPPGLEGKADRSEKSDGSGFGARLSPGSGGPEAQTAGPVTPASISGRFQPLPEAMKEKEVRPKHVKALLQMVRDESHQILALSEGLAPPSGEPHPPRKEDEIQDISLHGGKTQEVPTACPDWRGDLLQVVQEAFEKEQEMQGVELQPRLSGSDLGGHSSLLERLEKIIREQGDLQEKSLEHLRLPDRSSLLSEIQALRAQLRMTHLQNQEKLQHLRTALTSAEARGSQQEHQLRRQVELLAYKVEQEKCIAGDLQKTLSEEQEKANSVQKLLAAEQTVVRDLKSDLCESRQKSEQLSRSLCEVQQEVLQLRSMLSSKENELKAALQELESEQGKGRALQSQLEEEQLRHLQRESQSAKALEELRASLETQRAQSSRLCVALKHEQTAKDNLQKELRIEHSRCEALLAQERSQLSELQKDLAAEKSRTLELSEALRHERLLTEQLSQRTQEACVHQDTQAHHALLQKLKEEKSRVVDLQAMLEKVQQQALHSQQQLEAEAQKHCEALRREKEVSATLKSTVEALHTQKRELRCSLEREREKPAWLQAELEQSHPRLKEQEGRKAARRSAEARQSPAAAEQWRKWQRDKEKLRELELQRQRDLHKIKQLQQTVRDLESKDEVPGSRLHLGSARRAAGSDADHLREQQRELEAMRQRLLSAARLLTSFTSQAVDRTVNDWTSSNEKAVMSLLHTLEELKSDLSRPTSSQKKMAAELQFQFVDVLLKDNVSLTKALSTVTQEKLELSRAVSKLEKLLKHHLQKGCSPSRSERSAWKPDETAPQSSLRRPDPGRLPPAASEEAHTSNVKMEKLYLHYLRAESFRKALIYQKKYLLLLIGGFQDSEQETLSMIAHLGVFPSKAERKITSRPFTRFRTAVRVVIAILRLRFLVKKWQEVDRKGALAQGKAPRPGPRARQPQSPPRTRESPPTRDVPSGHTRDPARGRRLAAAASPHSGGRATPSPNSRLERSLTASQDPEHSLTEYIHHLEVIQQRLGGVLPDSTSKKSCHPMIKQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Horse
88
Dog
88
Chicken
71
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95613 作为底物

Site PTM Type Enzyme
S44 Phosphorylation
S54 Phosphorylation
S61 Phosphorylation
S175 Phosphorylation
S188 Phosphorylation
T191 Phosphorylation
S201 Phosphorylation
S253 Phosphorylation
S255 Phosphorylation
T259 Phosphorylation
K271 Ubiquitination
K273 Ubiquitination
K338 Acetylation
K354 Ubiquitination
K418 Ubiquitination
K437 Ubiquitination
S455 Phosphorylation
T479 Phosphorylation
K505 Ubiquitination
S514 Phosphorylation
K532 Ubiquitination
S556 Phosphorylation
S562 Phosphorylation
K569 Ubiquitination
S589 Phosphorylation
S610 Phosphorylation
S644 Phosphorylation
S682 Phosphorylation
K696 Ubiquitination
K788 Ubiquitination
S813 Phosphorylation
T815 Phosphorylation
S837 Phosphorylation
K881 Ubiquitination
K952 Ubiquitination
T962 Phosphorylation
K1004 Ubiquitination
S1006 Phosphorylation
K1018 Ubiquitination
K1022 Ubiquitination
K1069 Ubiquitination
K1088 Ubiquitination
S1142 Phosphorylation
K1145 Ubiquitination
T1178 Phosphorylation
T1209 Phosphorylation P53350 (PLK1)
T1221 Phosphorylation P53350 (PLK1)
S1235 Phosphorylation P53350 (PLK1)
S1241 Phosphorylation P53350 (PLK1)
S1245 Phosphorylation
S1289 Phosphorylation
K1293 Ubiquitination
S1296 Phosphorylation
K1298 Ubiquitination
K1308 Ubiquitination
K1317 Ubiquitination
K1341 Ubiquitination
K1348 Ubiquitination
S1367 Phosphorylation
S1430 Phosphorylation
K1437 Ubiquitination
K1472 Ubiquitination
K1535 Ubiquitination
K1550 Ubiquitination
S1632 Phosphorylation
S1653 Phosphorylation
S1671 Phosphorylation
S1688 Phosphorylation
S1693 Phosphorylation
S1703 Phosphorylation
K1705 Ubiquitination
R1710 Methylation
K1718 Ubiquitination
S1770 Phosphorylation
K1799 Ubiquitination
S1814 Phosphorylation
K1864 Ubiquitination
K1884 Ubiquitination
K2015 Ubiquitination
K2038 Ubiquitination
S2044 Phosphorylation
K2050 Ubiquitination
K2054 Ubiquitination
K2071 Ubiquitination
K2076 Ubiquitination
S2114 Phosphorylation
T2155 Phosphorylation
S2168 Phosphorylation
S2177 Phosphorylation
S2192 Phosphorylation
S2204 Phosphorylation
K2213 Acetylation
S2214 Phosphorylation
S2259 Phosphorylation
S2279 Phosphorylation
S2327 Phosphorylation
S2339 Phosphorylation
S2342 Phosphorylation
S2345 Phosphorylation
S2352 Phosphorylation
S2355 Phosphorylation
T2367 Phosphorylation
S2370 Phosphorylation
S2433 Phosphorylation
K2438 Ubiquitination
K2463 Ubiquitination
S2477 Phosphorylation
S2479 Phosphorylation
S2486 Phosphorylation
K2504 Ubiquitination
S2505 Phosphorylation
S2519 Phosphorylation
K2538 Ubiquitination
K2570 Ubiquitination
K2575 Ubiquitination
K2583 Ubiquitination
T2584 Phosphorylation
S2586 Phosphorylation
S2594 Phosphorylation
K2597 Ubiquitination
K2610 Ubiquitination
K2649 Ubiquitination
K2661 Ubiquitination
K2685 Ubiquitination
K2709 Acetylation
K2709 Ubiquitination
K2720 Ubiquitination
K2745 Ubiquitination
K2810 Ubiquitination
K2828 Ubiquitination
K2854 Ubiquitination
S2860 Phosphorylation
K2867 Ubiquitination
S2878 Phosphorylation
S2894 Phosphorylation
S2900 Phosphorylation
K2932 Ubiquitination
K2944 Ubiquitination
S2956 Phosphorylation
S2963 Phosphorylation
R2999 Methylation
K3010 Ubiquitination
S3014 Phosphorylation
K3023 Ubiquitination
K3034 Ubiquitination
K3035 Ubiquitination
K3057 Ubiquitination
K3066 Ubiquitination
S3070 Phosphorylation
S3074 Phosphorylation
K3075 Acetylation
K3075 Ubiquitination
K3081 Ubiquitination
K3086 Ubiquitination
K3099 Ubiquitination
S3108 Phosphorylation
K3131 Ubiquitination
Y3139 Phosphorylation
S3166 Phosphorylation
T3189 Phosphorylation
S3190 Phosphorylation
K3229 Ubiquitination
S3242 Phosphorylation
S3249 Phosphorylation
S3274 Phosphorylation
S3277 Phosphorylation
T3282 Phosphorylation
S3284 Phosphorylation
S3287 Phosphorylation
S3292 Phosphorylation
S3302 Phosphorylation
S3324 Phosphorylation
S3326 Phosphorylation
K3327 Ubiquitination
K3328 Ubiquitination

研究背景

功能:

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

翻译修饰:

Cleaved during mitotis which leads to removal of CDK5RAP2 from the centrosome and promotes centriole disengagement and subsequent centriole separation. The C-terminal fragment is rapidly degraded following cleavage.

细胞定位:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all tissues tested, including placenta, liver, kidney and thymus.

亚基结构:

Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity (By similarity). Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with isoform 1 of NEK2. Interacts with CEP131. Interacts with CCDC13. Interacts with CEP68. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.

蛋白家族:

Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.

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