Phospho-CPI17alpha (Thr38) Antibody - #AF3473

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产品: 磷酸化 CPI17alpha (Thr38) 抗体
货号: AF3473
描述: Rabbit polyclonal antibody to Phospho-CPI17alpha (Thr38)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit
蛋白号: Q96A00
RRID: AB_2834911

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-CPI17alpha (Thr38) Antibody detects endogenous levels of CPI17alpha only when phosphorylated at Threonine 38.
RRID:
AB_2834911
引用格式: Affinity Biosciences Cat# AF3473, RRID:AB_2834911.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;

抗原和靶标

免疫原:

A synthesized peptide derived from human CPI17alpha around the phosphorylation site of Thr38.

基因/基因ID:
PPP1R14A(94274)
描述:
PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.

研究领域

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

文献引用

1). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-CPI-17 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-CPI-17 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 vs. control group. CPI-17: C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa; p-CPI-17: phosphorylated C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa.
2). The potential role of the SIRT1-Nrf2 signaling pathway in alleviating hidden hearing loss via antioxidant stress. Cell biology international, 2025 (PubMed: 39618038) [IF=3.3]

Application: WB    Species: Mouse    Sample:

Figure 4 SIRT1/Nrf2 changes in response to oxidative stress. (a–c) Comparison of P-SIRT1 and SIRT1 expression levels in different groups. P-SIRT1 was normalized to SIRT1 expression, SIRT1 was normalized to β-actin expression. (d–f) Comparison of cytoplasmic Nrf2 and nuclear Nrf2 expression levels in different groups. (g) Nrf2 cytoplasmic nuclear translocation in different groups was detected via immunofluorescence staining using confocal microscopy. Scale bar = 50 μm. (h–l) Comparison of NQO1, HO-1, and Nrf2 protein expression levels in different groups. (m–o) Comparison of SOD activity, MDA, and T-AOC levels in different groups (p) Comparison of ATP production levels in different groups. (q, r) Mitochondrial membrane potential (MMP) fluorescence intensity in different groups using Rhodamine 123. n = 6, *p 

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