Phospho-CPI17alpha (Thr38) Antibody - #AF3473

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产品: 磷酸化 CPI17alpha (Thr38) 抗体
货号: AF3473
描述: Rabbit polyclonal antibody to Phospho-CPI17alpha (Thr38)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit
分子量: 22kDa; 17kD(Calculated).
蛋白号: Q96A00
RRID: AB_2834911

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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%)
克隆:
Polyclonal
特异性:
Phospho-CPI17alpha (Thr38) Antibody detects endogenous levels of CPI17alpha only when phosphorylated at Threonine 38.
RRID:
AB_2834911
引用格式: Affinity Biosciences Cat# AF3473, RRID:AB_2834911.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;

抗原和靶标

免疫原:
Uniprot:

Q96A00(PP14A_HUMAN) >>Visit HPA database.

基因/基因ID:
PPP1R14A(94274)
表达:
Q96A00 PP14A_HUMAN:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

描述:
PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.
序列:
MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
73
Horse
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96A00 作为底物

Site PTM Type Enzyme
R5 Methylation
S12 Phosphorylation O43293 (DAPK3)
S16 Phosphorylation
R19 Methylation
R21 Methylation
S26 Phosphorylation
K32 Methylation
T38 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA) , O75116 (ROCK2) , Q02156 (PRKCE) , O43293 (DAPK3) , Q15139 (PRKD1) , Q13976 (PRKG1) , P17252 (PRKCA) , Q13418 (ILK) , Q05513 (PRKCZ) , Q13153 (PAK1) , P98161 (PKD1)
K40 Methylation
S128 Phosphorylation
S130 Phosphorylation Q9UQM7 (CAMK2A)
S134 Phosphorylation
S136 Phosphorylation

研究背景

功能:

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

蛋白家族:

Belongs to the PP1 inhibitor family.

研究领域

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

文献引用

1). Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm-Induced Sudden Cardiac Death. International journal of molecular sciences, 2024 (PubMed: 38474189) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 3 Effects of CAS on the expression of phosphorylated CPI-17 in rat coronary arteries. (A,B) Representative photomicrographs and semi-quantitative data of p-CPI-17 protein levels detected via IHC staining. Lower enlarged images are from upper images. n = 6 per group. Scale bar: 100 µm (upper images) or 50 µm (lower images). (C) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in the coronary arteries of rats. Scale bar: 50 µm. (D) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 6 per group. (E,F) Western blot and quantitative analysis of p-CPI-17 protein levels of rat coronary arteries. n = 6 per group. Data presented as mean ± SEM. ** p < 0.01 vs. control group. CPI-17: C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa; p-CPI-17: phosphorylated C kinase-potentiated protein phosphatase 1 inhibitor of 17 kDa.

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